Figure 1
From: Molecular cloning and characterization of Escherichia coli K12 ygjG gene
The scheme of the ygjG gene and aer-ygjG intergenic region. A. The fragment (from -311 to +1480) containing ygjG gene (b3073) with aer-ygjG intergenic region, and sites for restriction enzymes used in cloning procedure are shown. The orientation of genes and location of σ54 promoter are represented by arrows. Nucleotides numbering is in relation to ygjG mRNA transcription initiation site designated as +1. ygjG upstream fragments fused with lacZ and subcloned into pBRP [23] vector are shown as bold lines. B. The sequence of ygjG upstream region. Nucleotides numbering as in part A. σ54 promoter, putative NRI and IHF binding sites, SD sequences and the first mRNA base are bold. The consensus sequences for IHF, NRI and σ54 promoter are shown above the main sequence. Two possible translation start codons are underlined and corresponding N-terminal amino acids are bold. The putative FIS binding site http://www.ncbi.nlm.nih.gov:80/ is overlined. The directions of ORF1 and ORF2 translation are shown by arrows.