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Table 3 Publications with positive/negative results on direct detection by PCR of C. pneumoniae in atheroscle-rotic tissue

From: Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair

Authors Year PCR method DNA purification Samples collected in PCR positive (%)
Wessely et al. [20] 2003 Semi-nested, 474-bp PstI fragment phenol/chloroform Germany 0/31 (0.0)
   Nested, MOMP gene    
Vainio et al. [21] 2002 Nested, MOMP gene QIAmp Tissue kit Norway 0/48 (0.0)
Ong et al. [22] 2001 Nested, MOMP gene QiAmp Tissue Kit Northern Ire 0/44 (0.0)
Meijer et al. [23] 2000 Single-step, 16S rRNA and MOMP genes Easy-DNA Kit, modified The Netherlands 0/13 (0.0)
Meijer et al. [24] 1999 Single-step, 16S rRNA and MOMP genes Easy-DNA Kit, modified The Netherlands 0/18 (0.0)
Palfrey et al. [15] 1999 Single-step, MOMP gene Gene Clean Kit England 0/8 (0.0)
Lindholt et al. [6] 1998 Nested, MOMP gene phenol/chloroform Denmark 0/124 (0.0)
Andreasen et al. [7] 1998 Multiplex, Chlamydia species Chelex 100 Denmark 0/22 (0.0)
Daus et al. [25] 1998 Semi-nested, 474-bp PstI fragment Proteinase K Germany 1*/29 (3.4)
Paterson et al. [26] 1998 Nested, omp1 gene Proteinase K Australia 0/49 (0.0)
Weiss et al. [27] 1996 Single-step, 16S rRNA gene Proteinase K Brooklyn, USA 0/58 (0.0)
Cochrane et al. [28] 2002 Single-step, 474-bp PstI fragment Qiamp Tissue Kit Australia 15/29 (51.7)
Valassina et al. [29] 2001 Single-step, omp1, 16S rDNA, Hsp70 gene Qiamp Tissue Kit Italy 13/58 (58.0)
Gutierrez et al. [30] 2001 Semi-nested, 474-bp PstI fragment High Pure PCR Template preparation Kit Spain 48/85 (56.5)
Farsak et al. [31] 2000 Single-step, 16S rDNA phenol/chloroform +CTAB Turkey 12/46 (26)
Jantos et al. [32] 1999 Single-step, omp1, 16S rDNA Proteinase K Germany 4/50 (8.0)
Wong et al. [33] 1999 Nested, omp1 phenol/chloroform England 26/68 (38)
Petersen et al. [34] 1998 Single-step, omp1 Qiamp Tissue Kit Sweden 14/40 (35.0)
Ouchi et al. [35] 1998 Nested, 474-bp PstI fragment phenol/chloroform Japan 16/29 (55.2)
Maass et al. [36] 1997 Nested, 474-bp PstI fragment phenol/chloroform +CTAB Germany 9/61 (14.8)
Ong et al. [37] 1996 Nested, MOMP gene phenol/chloroform England 19/43 (44.2)
Blasi et al. [38] 1996 Nested, 16S rDNA phenol/chloroform Italy 26/51 (51.0)
Campbell et al. [39] 1995 Single-step, 474-bp PstI fragment phenol/Chloro form or Qiamp USA 12/38 (31.6)
Kuo et al. [40] 1993 Single-step, 474-bp PstI fragment, 16S rDNA Phenol/chloroform South Africa 12/30 (40.0)
  1. *The single positive specimen was not positive on retesting
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BMC Microbiology

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