Table 3 Publications with positive/negative results on direct detection by PCR of C. pneumoniae in atheroscle-rotic tissue
Authors | Year | PCR method | DNA purification | Samples collected in | PCR positive (%) |
|---|---|---|---|---|---|
Wessely et al. [20] | 2003 | Semi-nested, 474-bp PstI fragment | phenol/chloroform | Germany | 0/31 (0.0) |
| Â | Â | Nested, MOMP gene | Â | Â | Â |
Vainio et al. [21] | 2002 | Nested, MOMP gene | QIAmp Tissue kit | Norway | 0/48 (0.0) |
Ong et al. [22] | 2001 | Nested, MOMP gene | QiAmp Tissue Kit | Northern Ire | 0/44 (0.0) |
Meijer et al. [23] | 2000 | Single-step, 16S rRNA and MOMP genes | Easy-DNA Kit, modified | The Netherlands | 0/13 (0.0) |
Meijer et al. [24] | 1999 | Single-step, 16S rRNA and MOMP genes | Easy-DNA Kit, modified | The Netherlands | 0/18 (0.0) |
Palfrey et al. [15] | 1999 | Single-step, MOMP gene | Gene Clean Kit | England | 0/8 (0.0) |
Lindholt et al. [6] | 1998 | Nested, MOMP gene | phenol/chloroform | Denmark | 0/124 (0.0) |
Andreasen et al. [7] | 1998 | Multiplex, Chlamydia species | Chelex 100 | Denmark | 0/22 (0.0) |
Daus et al. [25] | 1998 | Semi-nested, 474-bp PstI fragment | Proteinase K | Germany | 1*/29 (3.4) |
Paterson et al. [26] | 1998 | Nested, omp1 gene | Proteinase K | Australia | 0/49 (0.0) |
Weiss et al. [27] | 1996 | Single-step, 16S rRNA gene | Proteinase K | Brooklyn, USA | 0/58 (0.0) |
Cochrane et al. [28] | 2002 | Single-step, 474-bp PstI fragment | Qiamp Tissue Kit | Australia | 15/29 (51.7) |
Valassina et al. [29] | 2001 | Single-step, omp1, 16S rDNA, Hsp70 gene | Qiamp Tissue Kit | Italy | 13/58 (58.0) |
Gutierrez et al. [30] | 2001 | Semi-nested, 474-bp PstI fragment | High Pure PCR Template preparation Kit | Spain | 48/85 (56.5) |
Farsak et al. [31] | 2000 | Single-step, 16S rDNA | phenol/chloroform +CTAB | Turkey | 12/46 (26) |
Jantos et al. [32] | 1999 | Single-step, omp1, 16S rDNA | Proteinase K | Germany | 4/50 (8.0) |
Wong et al. [33] | 1999 | Nested, omp1 | phenol/chloroform | England | 26/68 (38) |
Petersen et al. [34] | 1998 | Single-step, omp1 | Qiamp Tissue Kit | Sweden | 14/40 (35.0) |
Ouchi et al. [35] | 1998 | Nested, 474-bp PstI fragment | phenol/chloroform | Japan | 16/29 (55.2) |
Maass et al. [36] | 1997 | Nested, 474-bp PstI fragment | phenol/chloroform +CTAB | Germany | 9/61 (14.8) |
Ong et al. [37] | 1996 | Nested, MOMP gene | phenol/chloroform | England | 19/43 (44.2) |
Blasi et al. [38] | 1996 | Nested, 16S rDNA | phenol/chloroform | Italy | 26/51 (51.0) |
Campbell et al. [39] | 1995 | Single-step, 474-bp PstI fragment | phenol/Chloro form or Qiamp | USA | 12/38 (31.6) |
Kuo et al. [40] | 1993 | Single-step, 474-bp PstI fragment, 16S rDNA | Phenol/chloroform | South Africa | 12/30 (40.0) |