|  |  | phenol/chloroform | phenol/chloroform + CTAB | DNeasy Tissue kit | Easy-DNA kit | Magna Pure | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | EB Dilution | Appr. EB conc. EB/μl | Mean output copies/μl | SD of Mean | % Rel. rec. | SD of % Relative rec. | Mean output copies/μl | SD of Mean | % Rel. rec. | SD of % Relative rec. | Mean output copies/μl | SD of Mean | % Rel. rec. | SD of % Relative rec. | Mean output copies/μl | SD of Mean | % Rel. rec. | SD of % Relative V | Mean output copies/μl | SD of Mean | % Rel. rec. | SD of % Relative rec. |
C. pneumoniae EB samples | negative | 0 | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - |
 | 1.2 × 10-8 | 0.2 | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - |
 | 1.2 × 10-6 | 20 | 3.76 | 4.60 | 42.8 | 52.4 | 1.38 | 0.410 | 15.8 | 4.7 | 8.36 | 3.59 | 95.3 | 40.9 | 0.464 | 0.160 | 5.3 | 1.8 | 8.78 | 0.005 | 100 | 0.06 |
 | 1.2 × 10-5 | 200 | 28.6 | 29.9 | 27.0 | 28.2 | 38.0 | 44.7 | 35.8 | 42.2 | 96.2 | 19.3 | 90.8 | 18.2 | 1.81 | 1.24 | 1.7 | 1.2 | 106 | 38.3 | 100 | 36.2 |
 | 1.2 × 10-4 | 2000 | 248 | 208 | 20.2 | 17.0 | 140 | 60.2 | 11.4 | 5.0 | 982 | 250 | 79.8 | 20.3 | 18.5 | 21.7 | 1.5 | 1.8 | 1.23 × 103 | 331 | 100 | 26.9 |
 | av rec % | - | - | - | 30.0 | 29.5 | - | - | 21.0 | 22.3 | - | - | 88.6 | 23.1 | - | - | 2.8 | 2.3 | - | - | 100 | 20.2 |
EB spiked aorta tissue samples | negative | 0 | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - |
 | 1.2 × 10-8 | 0.2 | 0 | 0 | 0 | 0 | 0.219 | 0.310 | 18.0 | 25.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.22 | 1.72 | 100 | 141.3 |
 | 1.2 × 10-6 | 20 | 10.1 | 3.92 | 53.2 | 20.7 | 14.3 | 7.05 | 75.6 | 37.3 | 13.9 | 4.82 | 73.7 | 25.5 | 2.33 | 0.184 | 12.3 | 1.0 | 18.9 | 2.86 | 100 | 15.1 |
 | 1.2 × 10-5 | 200 | 50.3 | 24.4 | 44.5 | 21.6 | 125 | 7.14 | 110.5 | 6.3 | 66.3 | 30.2 | 58.7 | 26.8 | 29.5 | 1.11 | 26.1 | 1.0 | 113 | 0.530 | 100 | 0.5 |
 | 1.2 × 10-4 | 2000 | 632 | 177 | 49.0 | 13.7 | 648 | 585 | 50.3 | 45.3 | 869 | 208 | 67.3 | 16.2 | 191 | 128 | 14.8 | 9.9 | 1.29 × 103 | 247 | 100 | 19.2 |
 | av rec % | - | - | - | 36.7 | 26.0 | - | - | 63.6 | 43.7 | - | - | 49.9 | 34.8 | - | - | 13.3 | 10.6 | - | - | 100 | 54.2 |
 | overall av rec % | - | - | - | 33.8 | 26.7 | - | - | 45.3 | 41.2 |  |  | 66.5 | 35.4 |  |  | 8.8 | 9.6 |  |  | 100 | 41.7 |