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Table 1 Five DNA extraction methods used on pure C. pneumoniae DNA and aorta tissue samples spiked with C. pneumoniae DNA For each sample type different DNA input concentrations were used, these are displayed in the first column. DNA input concentration was known as pure genomic C. pneumoniae DNA was used for preparation of samples. For each method and sample the mean output C. pneumoniae genomic DNA concentration and standard deviation (SD) is displayed for two replicates as determined with the quantitative pmp4 LightCycler PCR. In addition percentage of recovered DNA is calculated (% Rec.). Standard deviations (SD) are also shown for the recovery percentages.

From: Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair

   phenol/chloroform phenol/chloroform +CTAB DNeasy Tissue kit Easy-DNA kit Magna Pure
  Input copies /μl Mean output copies/μl SD of Mean % Rec. SD of % Rec. Mean output copies/μl SD of Mean % Rec. SD of % Rec. Mean output copies/μl SD of Mean % Rec. SD of % Rec. Mean output copies/μl SD of Mean % Rec. SD of % Rec. Mean output copies/μl SD of Mean % Rec. SD of % Rec.
Pure C. pneumoniae DNA samples 0 0 0 - - 0 0 - - 0 0 - - 0 0 - - 0 0 - -
  10 1.79 0.213 17.9 2.1 0 0 0.0 0 8.08 1.21 80.8 12.1 0.661 0.520 6.6 5.2 4.60 0.377 46.0 3.8
  103 296 15.4 29.6 1.5 11.8 3.20 1.2 0.3 658 77.7 65.8 7.8 48.9 14.5 4.9 1.4 508 49.0 50.8 4.9
  105 2.09 × 104 2.55 × 103 20.9 2.5 728 107 0.7 0.1 7.54 × 104 1.83 × 104 75.4 18.4 3.10 × 103 1.83 × 103 3.1 1.8 5.58 × 104 7.54 × 103 55.8 7.5
  av rec % - - 22.8 5.7 - - 0.6 0.6 - - 74.0 12.4 - - 4.9 3.0 - - 50.9 6.2
Aorta tissue samples spiked with C. pneumoniae DNA 0 0 0 - - 0 0 - - 0 0 - - 0 0 - - 0 0 -. -
  10 0 0 0.0 0 1.31 0.240 13.1 2.4 4.56 4.19 45.6 42.0 6.34 1.50 63.4 15.0 1.83 0.622 18.3 6.2
  103 70.8 32.7 7.1 3.3 13.0 6.46 1.3 0.6 285 23.9 28.5 2.4 255 13.7 25.5 1.4 82.0 6.08 8.2 0.6
  105 5.00 × 103 2.55 × 103 5.0 2.4 1.40 × 103 611 1.4 1.0 2.37 × 104 502 23.7 0.5 3.30 × 104 7.53 × 103 33.0 7.5 1.61 × 104 1.06 × 104 16.1 10.6
  av rec % - - 4.0 3.7 - - 5.3 6.2 - - 32.6 21.4 - - 40.6 19.4 - - 14.2 7.3
  over-all avrec % - - 13.4 10.8 - - 3.0 4.8 - - 53.3 27.3 - - 22.8 22.9 - - 32.5 20.2