Figure 1

Proteins synthesized in E. coli exposed to LVFX. (A) Exponentially-growing cells were incubated with 1, 2, or 10 μg/ml of LVFX for 10 min and then labeled with [³⁵S]-methionine and cysteine for 1 min at 30°C. Heat shock culture was prepared as described in Methods. (B) After incubation with 10 μg/ml of LVFX, aliquots (100 μl) of the cultures were taken at indicated times and labeled for 1 min at 30°C. Equal amounts of acid-precipitable counts were applied to each lane and the proteins were separated by SDS-PAGE. The gels were dried and then exposed to films (A and B). The protein bands alphabetized in (B) were quantified using a BAS2000A image analyzer and the relative rates of synthesis are shown in (C).