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Table 2 PCR primer sequences

From: Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs)

Primer name 5' to 3' sequence Reverse Primer
LOCUS-1 Fwd HEX·GGT GAT TGC CGC GTG GTT CCG TTG AAT GAG REV-3
LOCUS-2 Fwd HEX·CCC GCA TGA TCC GCG AAC AGC TGG ATG REV-1
LOCUS-3 Fwd NED·CAG GCG CTT GAG GAT GAG GCG GCA G REV-3
LOCUS-4 Fwd 6-FAM·GCA GAA TTT TCG AGG CAT TCG GCG ATG REV-3
LOCUS-5 Fwd 6-FAM·GTG CTC CAG GGC GCC GGG AGG TAT GTT TAG REV-3
LOCUS-6 Fwd NED·GCC GCA GGA AAG CAG GCG ATC TGG AGA TTA TC REV-3
LOCUS-7 Fwd 6-FAM·CAG AGC CGT CGG TGG TTA CTT GAG TAG GGC AG REV-1
LOCUS-8 Fwd NED·GTG GGA AGC GTT ATC CTT TAA CGG GAG TAA GGG REV-1
REV-1 GGG GAG TAT GTT TTG GTT GCG CAT GAC CGC --
REV-3 GGG GGC ART ARG GCA GTA TGT TAA GGG AAT AGG G --
  1. HEX, NED and 6-FAM are fluorescent dyes covalently bound to the 5' end of the synthetic primer.
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BMC Microbiology

ISSN: 1471-2180

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