Table I Efficiency of transformation of deletions into TIGR4
Target gene | homology upstream* | homology downstream | size of inserted marker | size of deletion | trans-forming DNA (ng/ml) | trans-formants (CFU/ ml) | selected trans-formant |
|---|---|---|---|---|---|---|---|
SP0071 – zmp C | 757 (including 27 bp of zmp C) | 807 (including 1 bp of zmp C) | 1184 (cat) | 5543 | 500 | 100 | FP122 |
SP0664 – zmp B | 715 (including 25 bp of zmpB) | 838 (from 7 bp after zmp B) | 894 (erm B) | 5628 | 1500 | 10 | FP173 |
SP1154 – iga | 801 (including 11 bp of iga) | 828 (including 27 bp of iga) | 850 (spe) | 6004 | 1500 | 120 | FP174 |