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Table I Efficiency of transformation of deletions into TIGR4

From: The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice

Target gene homology upstream* homology downstream size of inserted marker size of deletion trans-forming DNA (ng/ml) trans-formants (CFU/ ml) selected trans-formant
SP0071 – zmp C 757 (including 27 bp of zmp C) 807 (including 1 bp of zmp C) 1184 (cat) 5543 500 100 FP122
SP0664 – zmp B 715 (including 25 bp of zmpB) 838 (from 7 bp after zmp B) 894 (erm B) 5628 1500 10 FP173
SP1154 – iga 801 (including 11 bp of iga) 828 (including 27 bp of iga) 850 (spe) 6004 1500 120 FP174
  1. * all sizes are expressed in base pairs. The total number of transformants obtained transforming 1 μg/ml were 5.2 × 102 CFU/ml using a chromosomal DNA carrying a point mutation conferring novobiocin resistance (positive control).