Figure 4From: Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosisKinetic characterisation of M. smegmatis methionine adenosyltransferase. The enzyme was incubated with 0 – 4.0 mM substrate and 10 mM cosubstrate as described in the Methods section. The production of SAM was measured by HPLC, and the resulting data fitted to the Michaelis-Menton equation.Back to article page