Immunoblots Probed With High Titred Antiserum Pools. SDS PAGE was run with samples which were not reduced with 2-mercaptoethanol and not heated before electrotransfer to nitrocellulose membranes. Immunoblots were probed with (A): convalescent dengue serum (HPR) and (B): JEV positive swine serum (PPJ). Lane C contains uninfected cell lysates while Lanes 1, 2, 3, 4 and 5 contain lysates of DENV1, 2, 3, 4 and JEV respectively. The position for bands of NS1 dimer, E and prM are labeled on the right.