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Table 3 Bacterial strains and plasmids used in this study.

From: The methionine salvage pathway in Bacillus subtilis

Strain or plasmid Genotype or description Source or reference
Strains   
Escherichia coli   
   TG1 K12 supE hsd Δ5 thi Δ (lac-proAB) F' [traD 36 proA+ proB+ lacIqlacZ ΔM15] Laboratory collection
   XL1-Blue K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi relA 1 lac- F' [proAB+ lacIqlacZ ΔM15 Tn10(tetR)] Laboratory collection
Bacillus subtilis   
   168 trpC 2 [31]
BSIP1143 trpC 2 metI::spc [32]
   BSHP7010 trpC 2 mtnS::spc [6]
   BFS1850 trpC 2mtnK::lacZ Functional analysis projecta[6]
   BFS1851 trpC 2mtnU::lacZ Functional analysis projecta[6]
   BFS1852 trpC 2mtnX::lacZ Functional analysis projecta
   BFS1853 trpC 2mtnZ::lacZ Functional analysis projecta
   BSHP7014 trpC 2mtnW::lacZ amyE::(pxylmtnXYZ) This work
   BSHP7015 trpC 2amyE::(pxylmtnXYZ) This work
   BSHP7016 trpC 2mtnY::lacZ This work
   BSHP7020 trpC 2mtnV::lacZ This work
   BSHP7064 trpC 2mtnW::Tn10 This work
   BSHP7065 trpC 2mtnY::Tn10 This work
Plasmids   
   pIC333 mini-Tn10 delivery vector, SpcR, EryR [27]
   pJM783 cloning vector, CmR, AmpR [25]
   pX cloning vector, CmR, AmpR, pxyl promoter, amyE locus integration [24]
   pHPP7011 pJM mtnV::lacZ This work
   pHPP7014 pJM mtnW::lacZ This work
   pHPP7014bis pJM mtnW::lacZ (bla::spcb) This work
   pHPP7015 pX pxyl mtnXYZ This work
   pHPP7016 pJM mtnY::lacZ This work
  1. a. This strain has been constructed in the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe. b. spc is the spectinomycin resistance gene from Staphylococcus aureus.