Table 3 Bacterial strains and plasmids used in this study.

Strains

Escherichia coli

   TG1

K12 supE hsd Δ5 thi Δ (lac-proAB) F' [traD 36 proA+ proB+ lacI^qlacZ ΔM15]

Laboratory collection

   XL1-Blue

K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi relA 1 lac- F' [proAB+ lacI^qlacZ ΔM15 Tn10(tet^R)]

Laboratory collection

Bacillus subtilis

   168

trpC 2

[31]

BSIP1143

trpC 2 metI::spc

[32]

   BSHP7010

trpC 2 mtnS::spc

[6]

   BFS1850

trpC 2mtnK::lacZ

Functional analysis project^a[6]

   BFS1851

trpC 2mtnU::lacZ

Functional analysis project^a[6]

   BFS1852

trpC 2mtnX::lacZ

Functional analysis project^a

   BFS1853

trpC 2mtnZ::lacZ

Functional analysis project^a

   BSHP7014

trpC 2mtnW::lacZ amyE::(pxylmtnXYZ)

This work

   BSHP7015

trpC 2amyE::(pxylmtnXYZ)

This work

   BSHP7016

trpC 2mtnY::lacZ

This work

   BSHP7020

trpC 2mtnV::lacZ

This work

   BSHP7064

trpC 2mtnW::Tn10

This work

   BSHP7065

trpC 2mtnY::Tn10

This work

Plasmids

   pIC333

mini-Tn10 delivery vector, Spc^R, Ery^R

[27]

   pJM783

cloning vector, Cm^R, Amp^R

[25]

   pX

cloning vector, Cm^R, Amp^R, pxyl promoter, amyE locus integration

[24]

   pHPP7011

pJM mtnV::lacZ

This work

   pHPP7014

pJM mtnW::lacZ

This work

   pHPP7014bis

pJM mtnW::lacZ (bla::spc^b)

This work

   pHPP7015

pX pxyl mtnXYZ

This work

   pHPP7016

pJM mtnY::lacZ

This work