Figure 3

Northern blot analysis of B. subtilis 168 mtnVWXYZ region. A total of 3 μg of RNA was used. A. Northern hybridization with mtnV gene specific probe. RNA corresponding to lane 1 was obtained from a culture grown in minimal medium with sulfate as a sulfur source, and for lane 2 from a culture grown in minimal medium with methionine as a sulfur source. B. Northern hybrydization with mtnW gene specific probe. RNA corresponding to lane 1 was obtained from a culture grown in minimal medium with sulfate as a sulfur source, and for lane 2 from a culture grown in minimal medium with methionine as a sulfur source. C. Northern hybrydization with mtnZ gene specific probe. RNA corresponding to lane 1 was obtained from a culture grown in minimal medium with sulfate as a sulfur source, and for lane 2 from a culture grown in minimal medium with methionine as a sulfur source.