Figure 2

Identification of the mtn region promoters by primer extension. A. Identification of the transcription start site of the mtnU operon. The size of the extended product is compared to a DNA-sequencing ladder of the mtnU promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. B. Identification of the transcription start site of the mtnV gene. The size of the extended product is compared to a DNA-sequencing ladder of the mtnV promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. C. Identification of the transcription start site of the mtnKS gene. The size of the extended product is compared to a DNA-sequencing ladder of the mtnKS promoter region. Primer extension and sequencing reaction were performed with the same primer. The +1 site is marked by an arrow. D. Identification of the transcription start site of the mtnWXYZ operon. The size of the extended product is compared to a DNA-sequencing ladder of the mtnWXYZ promoter region. Primer extension and sequencing reaction were performed with the same primer. Two +1 sites are marked by arrows. E. Sequences of the corresponding promoter regions. Promoter sites are in capital letters and underlined (-35 and -10 boxes), and the transcription start sites are indicated by broken arrows (+1).