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Table 1 Library of ARDRA profiles (combination of restriction patterns) obtained for mycobacterial species.

From: Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory

Species Genbank Number Hha I Mbo I Rsa I Bst UI Reference strains useda
M. tuberculosis complex X52917 1 1 1 1 ITG 8021, IPB 92/0805
M. conspicuum X88922 1' 1 2 2  
M. intracellulare X52927 1' 2 2 3 ITG 5913, ITG 5917
M. gastri X52919 1' 4 1 1  
M. kansasii M29575 1' 4 1 1 ITG 8201
M. bohemicum AJ277283 1' 4 1 3  
M. haemophilum U06638 1' 4 1 3 ITG 3065
M. malmoense AF152560 1' 4 1 3 ITG 940611
M. szulgai X52926 1' 4 2 1 ITG 4981
M. scrofulaceum X52924 1' 4 2 3 ITG 4988
M. xenopi X52929 1' 4 3 2 ITG 4986
M. heckeshornense AF174290 1' 4 3 4  
M. marinum AF251565 2 1 1 1 ITG 1728
M. asiaticum M29556 2 1 2 5 ITG 8182
M. terrae X52925 2 1 4 2 ITG 4922
M. ulcerans Z13990 2 1 9 1 ITG 724, ITG 1837
M. avium AF306455 2 2 2 3 ITG 4991, ITG 2666
M. botniense AJ012756 2 4 3 2  
M. terrae-like MCRO6b X93032 2 6 4 7  
M. nonchromogenicum X52928 2 6 4 7 ITG 4980
M. phlei M29566 3 1 6 2'  
M. elephantis AJ010747 3 1 8 3'  
M. chelonae group IVc AJ416940 3 3 5 2' ITG 7701
M. abscessus group IIIc AJ419970 3 3 6 2' ITG 98-1296
M. chelonae group 1c AJ419969 3 3 6 2' ITG 95-0026
M. immunogenum AJ011771 3 3 6 2' ITG 98-1289
M. farcinogenes AF055333 4 1 6 7  
M. fortuitum X52933 4 1 6 7 SLZ A046
M. senegalense M29567 4 1 6 7  
M. septicum AF111809 4 1 6 7 ITG 4166
M. intermedium X67847 5 4 6 1'  
M. interjectum AJ272088 5 4 6 2' ITG 96-116
M. heidelbergense X70960 5 4 8 2'  
M. simiae X52931 5 7 6 2' ITG 4485
M. lentiflavum X80769 5 7 6 2'  
M. peregrinum d X52921 7 1 6 9 ITG 99-2069, ATCC 14467
M. gordonae M29563 8 4 2 5 ITG 7838
M. wolinskyi Y12871 10 1 1 2'  
M. species a AF028712 10 1 1 2'  
M. goodii Y12872 10 1 2 2'  
M. smegmatis AJ131761 10 1 2 2' ITG 4995
M. kubicae AF133902 10 1 6 1'  
M. tusciae AF058299 10 1 6 2'  
M. flavescens X52932 10 1 6 2' VUB A016
M. genavense X60070 10 7 6 2' ITG 97-102
M. triplex U57632 10 7 6 2'  
M. chelonae group IIc AJ419968 13 3 6 6 ITG 96-0295
M. branderi X82234 14 1 1 9  
M. celatum e L08169 14 1 1 10  
M. triviale X88924 15 1 10 10'  
M. aurum X55595 16 1 6 8  
  1. a. ITG: Institute for Tropical Medicine, Antwerp, Belgium; IPB: Institute Pasteur du Brabant, Brussels, Belgium; SLZ: Streeklaboratorium Zeeland, Goes, the Netherlands; VUB: Free University of Brussels, Brussels, Belgium. b. Strains designated MCRO6 have been studied by Turenne et al.[22] and Torkko et al.[33]. c. Sequences determined in this study. Roman numbering according to Portaels et al.[16], who distinguished four groups within the M. abscessus/M. chelonae complex, based on the 16S–23S rRNA spacer region. d. The GenBank sequence (AF058712) of strain ATCC 14467, submitted to GenBank as M. peregrinum, did not cluster within the M. fortuitum complex (Figure 8) and was highly similar to sequence Y12871 (M. wolinskyi). Moreover, the ARDRA profile calculated from sequence AF058712 (10-1-1-2') did not correspond with the profile we obtained for strain ATCC 14467 (4-1-6-7). The sequence obtained in this study from strain ATCC 14467 (submitted as AJ422046) was identical to the M. fortuitum sequence X52933. Sequence AF058712 is indicated as M. species in the table. e. Received as M. xenopi.