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Table 2 Set of primers for MLVA analysis

From: High resolution, on-line identification of strains from the Mycobacterium tuberculosis complex based on tandem repeat typing

Locus name

forward primer

reverse primer

H37Rv_0024_18 bp

GAGAAACAGGAGGGCGTTG

TATTACGACGACCGCTATGC

H37Rv_0079_9 bp

CGTGCACAGTTGGGTGTTTA

TTCGTTCAGGAACTCCAAGG

H37Rv_0154_53 bp

TGGACTTGCAGCAATGGACCAACT

TACTCGGACGCCGGCTCAAAAT

H37Rv_0424_51 bp

GTCCAGGTTGCAAGAGATGG

GGCATCCTCAACAACGGTAG

H37Rv_0531_15 bp

GGTTACCACTTCGATGCGTCTGCG

AGCCGCCGAAACCCATC

H37Rv_0577_58 bp*

GACTTCAATGCGTTGTTGGA*

GTCTTGACCTCCACGAGTGC*

H37Rv_0580_77 bp

CAGGTCACAACGAGAGGAAGAGC

GCGGATCGGCCAGCGACTCCTC

H37Rv_0802_54 bp*

AAGCGCAAGAGCACCAAG*

GTGGGCTTGTACTTGCGAAT*

H37Rv_0959_53 bp

GTTCTTGACCAACTGCAGTCGTCC

GCCACCTTGGTGATCAGCTACCT

H37Rv_1121_15 bp

CTCCCACACCCAGGACAC

CGGCCTACCCAACATTCC

H37Rv_1443_56 bp

GGTAATCCTGGTCGCTTGTC

ACCCAAATTGCCCTGGTC

H37Rv_1451_57 bp

GGTAGCCGTCGTCGAGAAGC

CGCCACCACCGCACTGGC

H37Rv_1612_21 bp

GCTGCACCGGTGCCCATC

CACCGGAGCCGGAACGGC

H37Rv_1644_53 bp

TCGGTGATCGGGTCCAGTCCAAGTA

CCCGTCGTGCAGCCCTGGTAC

H37Rv_1895_57 bp

GGTGCACGGCCTCGGCTCC

AAGCCCCGCCGCCAATCAA

H37Rv_1955_57 bp

AGATCCCAGTTGTCGTCGTC

CAACATCGCCTGGTTCTGTA

H37Rv_1982_78 bp*

ATCGTCAGCTGCGGAATAGT*

AATACCGGGGATATCGGTTC*

H37Rv_2059_77 bp

TCGGAGAGATGCCCTTCGAGTTAG

GGAGACCGCGACCAGGTACTTGTA

H37Rv_2074_56 bp

AAATTCAAAGAGTTTCTCGACAGTG

GATCTTGAGAACCAAGATGTCCTT

H37Rv_2163_a_69 bp

CCCATCCCGCTTAGCACATTCGTA

TTCAGGGGGGATCCGGGA

H37Rv_2163_b_69 bp

CGTAAGGGGGATGCGGGAAATAGG

CGAAGTGAATGGTGGCAT

H37Rv_2165_75pb*

ATTTCGATCGGGATGTTGAT*

TCGGTCCCATCACCTTCTTA*

H37Rv_2347_57 bp

AACCCATGTCAGCCAGGTTA

ATGATGGCACACCGAAGAAC

H37Rv_2401_58 bp

AGTCACCTTTCCTACCACTCGTAAC

ATTAGTAGGGCACTAGCACCTCAAG

H37Rv_2461_57 bp

GCGAACACCAGGACAGCATCATG

GGCATGCCGGTGATCGAGTGG

H37Rv_2531_53 bp

CAGCGAAACGAACTGTGCTATCAC

CGTGTCCGAGCAGAAAAGGGTAT

H37RV_2387_54 bp

CGACCAAGATGTGCAGGAATACAT

GGGCGAGTTGAGCTCACAGAA

H37Rv_2990_55 bp

GTGACGTTTACCGTGCTCTATTTC

GTCGTCGGACAGTTCTAGCTTT

H37Rv_2996_51 bp

CCCGCCTTCGAAACGTCGCT

TGGACATAGGCGACCAGGCGAATA

H37Rv_3006_53 bp

TCGAAAGCCTCTGCGTGCCAGTAA

GCGATGTGAGCGTGCCACTCAA

H37Rv_3171_54 bp

GCAGATAACCCGCAGGAATA

GGAGAGGATACGTGGATTTGAG

H37Rv_3192_53 bp*

ACTGATTGGCTTCATACGGCTTTA*

GTGCCGACGTGGTCTTGAT*

H37Rv_3232_56 bp

CAGACCCGGCGTCATCAAC

CCAAGGGCGGCATTGTGTT

H37Rv_3239_79 bp

CTCGGTGATGGTCCGGCCGGTCAC

GGAAGTGCTCGACAACGCCATGCC

H37Rv_3336_59 bp

ATCCCCGCGGTACCCATC

GCCAGCGGTGTCGACTATCC

H37Rv_3663_63 bp

GCCCAAAAAGCATGGGAACGTGCCCCT

GGTTGTCCCCGCAGTATCTC

H37Rv_3690_58 bp

AATCACGGTAACTTGGGTTGTTT

GATGCATGTTCGACCCGTAG

H37Rv_4052_111 bp

AACGCTCAGCTGTCGGAT

GGCCAGGTCCTTCCCGAT

H37Rv_4156_59 bp*

TGGTCGCTACGCATCGTGTCGGCCCGT*

TACCACCCGGGCAGTTTAC*

H37Rv_4348_53 bp

CGCATCGACAAACTGGAGCCAAAC

CGGAAACGTCTACGCCCCACACAT

  1. * : the primers indicated are not the primers used in the princeps publication, but were designed for the present study, usually in order to reduce the size of the PCR product and consequently to improve allele size identification.
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BMC Microbiology

ISSN: 1471-2180

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