The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029

Table 1 Overview of Pmps, antibodies and immunoblot reactions

pAb	Pmp	Cl.	Fam.	L	aa	cloned	MS	G	M	IMB	MeOH	Form.
204	1	I	G	I	923	26–923	-	-	I	1?2 6 78 10 13 14 20 21	X	-
207	2	I	G	I	842	20–842	X	S	M	2 6 8 10	X	-
-	3*	I	G	I
-	4*	I	G	I
-	5*	I	G	I
221	6	II	G	I	1408	18–905	N+C	S	M	6 8 10X	X	X
220	7	II	G	I	937	25–545	X	S		2 6 7 8 10 11 13	X	-
201	8	II	G	I	931	21–931	X	S	M	8 10	X	X
208	9	II	G	I	929	27–929	-	S	I	2 6 7 8 9? 10 11 13 14 20	X	u
203	10	II	G	II	929	20–929	X	-	M	10	X	X
195	11	II	G	II	929	1–929	X	W	M	6 11	X	X
-	12#	II	-	I
222	13	II	G	I	974	20–542	X	S	M	not performed	X	-
228	14	II	H	I	979	25–492	X	S	M	not performed	X	-
229	15	III	EF	I	939	18–478	-	W	I	-	-	-
230	16	III	EF	N	935	1–472	-	S	M	8 10	-	-
-	17*	III	EF	I
231	18	III	EF	N	893	1–566	-	P		8 10	-	-
234	19	IV	A	I	948	22–501	-	-		-	-	-
236	20	IV	BC	I	1724	22–1272	X	-	M	20 13	-	-
237	21		D	I	1610	52–1129	N+C	-	M	6 13Y	X	X

pAb: Antibody number; Pmp: Pmp number: *, genes containing a premature stop; #, naturally short gene; shading indicates either * or #. Cl.: Cluster in the C. pneumoniae genome.L: Predicted leader sequences: I, signal peptidase I cleavage site; II, signal peptidase II cleavage site; -, no predicted cleavage site. Fam.: Closest related C. trachomatis Pmp. aa: Amino acids in full-length Pmp. cloned: First and last amino acid in the recombinant protein. MS: Mass spectrometry identification by: X, peptides dispersed along the entire sequence; N, peptides localized to the N-terminal part; C, peptides localized to the C-terminal part; -, not identified by MS. G: Detection in 1D IMB by Grimwood et al.: S, strong reaction; W, weak reaction; P, possible reaction; -, no reaction. M: Detection by Montigiani et al. [24]: M, MS and immunoblot identification; I, immunoblot detection alone. IMB: Immunoblotting results: bold numbers indicate strong reaction; X is a C-terminal fragment of Pmp6; Y is an N-terminal fragment of Pmp21; results marked ? were inconclusive. MeOH: MeOH fixation IMF at 72 hpi: X, reaction; -, no reaction. Form.: Formalin fixation IMF at 72 hpi: X specific reaction; u, unspecific reaction; -, no reaction.

ISSN: 1471-2180