|
pAb
|
Pmp
|
Cl.
|
Fam.
|
L
|
aa
|
cloned
|
MS
|
G
|
M
|
IMB
|
MeOH
|
Form.
|
|---|
|
204
|
1
|
I
|
G
|
I
|
923
|
26–923
|
-
|
-
|
I
|
1?2 6 78 10 13 14 20 21
|
X
|
-
|
|
207
|
2
|
I
|
G
|
I
|
842
|
20–842
|
X
|
S
|
M
|
2 6 8 10
|
X
|
-
|
|
-
|
3*
|
I
|
G
|
I
| | | | | | | | |
|
-
|
4*
|
I
|
G
|
I
| | | | | | | | |
|
-
|
5*
|
I
|
G
|
I
| | | | | | | | |
|
221
|
6
|
II
|
G
|
I
|
1408
|
18–905
|
N+C
|
S
|
M
|
6 8 10X
|
X
|
X
|
|
220
|
7
|
II
|
G
|
I
|
937
|
25–545
|
X
|
S
| |
2 6 7 8 10 11 13
|
X
|
-
|
|
201
|
8
|
II
|
G
|
I
|
931
|
21–931
|
X
|
S
|
M
|
8 10
|
X
|
X
|
|
208
|
9
|
II
|
G
|
I
|
929
|
27–929
|
-
|
S
|
I
|
2 6 7 8 9? 10 11 13 14 20
|
X
|
u
|
|
203
|
10
|
II
|
G
|
II
|
929
|
20–929
|
X
|
-
|
M
|
10
|
X
|
X
|
|
195
|
11
|
II
|
G
|
II
|
929
|
1–929
|
X
|
W
|
M
|
6 11
|
X
|
X
|
|
-
|
12#
|
II
|
-
|
I
| | | | | | | | |
|
222
|
13
|
II
|
G
|
I
|
974
|
20–542
|
X
|
S
|
M
|
not performed
|
X
|
-
|
|
228
|
14
|
II
|
H
|
I
|
979
|
25–492
|
X
|
S
|
M
|
not performed
|
X
|
-
|
|
229
|
15
|
III
|
EF
|
I
|
939
|
18–478
|
-
|
W
|
I
|
-
|
-
|
-
|
|
230
|
16
|
III
|
EF
|
N
|
935
|
1–472
|
-
|
S
|
M
|
8 10
|
-
|
-
|
|
-
|
17*
|
III
|
EF
|
I
| | | | | | | | |
|
231
|
18
|
III
|
EF
|
N
|
893
|
1–566
|
-
|
P
| |
8 10
|
-
|
-
|
|
234
|
19
|
IV
|
A
|
I
|
948
|
22–501
|
-
|
-
| |
-
|
-
|
-
|
|
236
|
20
|
IV
|
BC
|
I
|
1724
|
22–1272
|
X
|
-
|
M
|
20 13
|
-
|
-
|
|
237
|
21
| |
D
|
I
|
1610
|
52–1129
|
N+C
|
-
|
M
|
6 13Y
|
X
|
X
|
- pAb: Antibody number; Pmp: Pmp number: *, genes containing a premature stop; #, naturally short gene; shading indicates either * or #. Cl.: Cluster in the C. pneumoniae genome.L: Predicted leader sequences: I, signal peptidase I cleavage site; II, signal peptidase II cleavage site; -, no predicted cleavage site. Fam.: Closest related C. trachomatis Pmp. aa: Amino acids in full-length Pmp. cloned: First and last amino acid in the recombinant protein. MS: Mass spectrometry identification by: X, peptides dispersed along the entire sequence; N, peptides localized to the N-terminal part; C, peptides localized to the C-terminal part; -, not identified by MS. G: Detection in 1D IMB by Grimwood et al.: S, strong reaction; W, weak reaction; P, possible reaction; -, no reaction. M: Detection by Montigiani et al. [24]: M, MS and immunoblot identification; I, immunoblot detection alone. IMB: Immunoblotting results: bold numbers indicate strong reaction; X is a C-terminal fragment of Pmp6; Y is an N-terminal fragment of Pmp21; results marked ? were inconclusive. MeOH: MeOH fixation IMF at 72 hpi: X, reaction; -, no reaction. Form.: Formalin fixation IMF at 72 hpi: X specific reaction; u, unspecific reaction; -, no reaction.
