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Table 1 Overview of Pmps, antibodies and immunoblot reactions

From: The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029

pAb Pmp Cl. Fam. L aa cloned MS G M IMB MeOH Form.
204 1 I G I 923 26–923 - - I 1?2 6 78 10 13 14 20 21 X -
207 2 I G I 842 20–842 X S M 2 6 8 10 X -
- 3* I G I         
- 4* I G I         
- 5* I G I         
221 6 II G I 1408 18–905 N+C S M 6 8 10X X X
220 7 II G I 937 25–545 X S   2 6 7 8 10 11 13 X -
201 8 II G I 931 21–931 X S M 8 10 X X
208 9 II G I 929 27–929 - S I 2 6 7 8 9? 10 11 13 14 20 X u
203 10 II G II 929 20–929 X - M 10 X X
195 11 II G II 929 1–929 X W M 6 11 X X
- 12# II - I         
222 13 II G I 974 20–542 X S M not performed X -
228 14 II H I 979 25–492 X S M not performed X -
229 15 III EF I 939 18–478 - W I - - -
230 16 III EF N 935 1–472 - S M 8 10 - -
- 17* III EF I         
231 18 III EF N 893 1–566 - P   8 10 - -
234 19 IV A I 948 22–501 - -   - - -
236 20 IV BC I 1724 22–1272 X - M 20 13 - -
237 21   D I 1610 52–1129 N+C - M 6 13Y X X
  1. pAb: Antibody number; Pmp: Pmp number: *, genes containing a premature stop; #, naturally short gene; shading indicates either * or #. Cl.: Cluster in the C. pneumoniae genome.L: Predicted leader sequences: I, signal peptidase I cleavage site; II, signal peptidase II cleavage site; -, no predicted cleavage site. Fam.: Closest related C. trachomatis Pmp. aa: Amino acids in full-length Pmp. cloned: First and last amino acid in the recombinant protein. MS: Mass spectrometry identification by: X, peptides dispersed along the entire sequence; N, peptides localized to the N-terminal part; C, peptides localized to the C-terminal part; -, not identified by MS. G: Detection in 1D IMB by Grimwood et al.: S, strong reaction; W, weak reaction; P, possible reaction; -, no reaction. M: Detection by Montigiani et al. [24]: M, MS and immunoblot identification; I, immunoblot detection alone. IMB: Immunoblotting results: bold numbers indicate strong reaction; X is a C-terminal fragment of Pmp6; Y is an N-terminal fragment of Pmp21; results marked ? were inconclusive. MeOH: MeOH fixation IMF at 72 hpi: X, reaction; -, no reaction. Form.: Formalin fixation IMF at 72 hpi: X specific reaction; u, unspecific reaction; -, no reaction.