Figure 1

2-D immunoblotting of EB proteins Radiolabeled proteins from EBs purified 72 hpi were separated by 2-D PAGE and electroblotted onto PVDF membranes. The membranes were reacted with polyclonal rabbit antibodies raised against recombinant Pmp. Bound antibodies were visualized by color reagents and membranes were afterwards exposed to X-ray films so that reacting spots could be identified by comparison to an annotated gel. A: PVDF blot of EB proteins reacted with Pmp6-pAb221, X shows a C-terminal fragment of Pmp6 recognized by Pmp6-pAb221; B: Autoradiography of A; C: PVDF blot of EB proteins reacted with pAb237-rPmp21, Y shows an N-terminal fragment of Pmp21 recognized by pAb237-rPmp21.