Figure 2From: Recognition of secretory proteins in Escherichia coli requires signals in addition to the signal sequence and slow foldingImmunoprecipitations of SecB complexes A. Precipitation was performed with antibody directed against SecB, followed by immunoblotting using antibody against the His tag on the λ repressor fragments. B. Precipitating antibody was directed against the His tag, immunoblotting utilized the anti-SecB antibody. C. Precipitation was carried out with antibody against LamB, anti-SecB was used for immunoblotting. In all gels, samples were as follows: lanes 1–4 – whole cell lysates prior to immunoprecipitation, lanes 5–8 – immunoprecipitated material; lanes 1 and 5 – N102LT/prlA4, lanes 2 and 6 – LA57-N102LT/prlA4, lanes 3 and 7 – N102LT/prlA+, lanes 4 and 8 – LA57-N102LT/prlA+, lane 9 in gel B – purified SecB.Back to article page