Figure 5
From: Detection and characterization of the S. typhimurium HilA protein
Subcellular fractionation of HilA proteins Proteins from cleared cell lysates (lanes 1 and 4), soluble fractions (lanes 2 and 5), and membrane pellet fractions (lanes 3 and 6) were detected by Western analysis using polyclonal antiserum raised against HilA-His. A. Native HilA expressed in S. typhimurium strain EE656 grown in high osmolarity, low aeration conditions for 3 hours. B. HilA-Myc-His expressed in E. coli DH5α/pCH112. Samples harvested one hour after induction with arabinose (lanes 1,2,3) vs. two hours after induction with arabinose (lanes 4,5,6).