Figure 4
From: Detection and characterization of the S. typhimurium HilA protein
Mobility of native HilA and engineered forms of HilA The migration of proteins from wild-type S. typhimurium SL1344 grown for 3 hours in high osmolarity, low aeration conditions (lane 2) is compared to that of a 553 amino acid (63 kDa) derivative of HilA produced by pCR53 (lane 1) and to that of a 575 amino acid (65.5 kDa) HilA-Myc-His derivative produced by pCH112 (lane 3). 50-fold less of the pCH112 and pCR53 samples were run on the gel, as compared to the SL1344 sample, to compensate for the higher level of HilA produced from the arabinose-inducible plasmid constructs. Western analysis was conducted using polyclonal antiserum raised against HilA-His.