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Figure 3 | BMC Microbiology

Figure 3

From: Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction)

Figure 3

LDR detection on the Universal Microarray with defined templates. Each row of the array corresponds to a group and contains ten-spot replicates. Deposition scheme: 1, Zip 3 (bacilli,) 2, Zip 5 (myxobacteria); 3, Zip 11 (cyanobacteria); 4, Zip 13 (universal); 5, Zip 15 (pseudomonads); 6, Zip 21 (actinomycetes). Zip sequences are from Barany et al (6). Panel A shows the results obtained after hybridization to Cy5 poly(dT)10. Panel B through H show the results of hybridization after LDR on 16S rDNA templates from bacilli (panel B), myxobacteria (panel C), cyanobacteria (panel D), pseudomonads (panel E), actinomycetes (panel F), C. perfringens (panel G) and E. coli (panel H). All the images were acquired setting both the Laser power and PMT gain to 85%

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BMC Microbiology

ISSN: 1471-2180

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