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Figure 2 | BMC Microbiology

Figure 2

From: HIV-1 expression induces cyclin D1 expression and pRb phosphorylation in infected podocytes: cell-cycle mechanisms contributing to the proliferative phenotype in HIV-associated nephropathy

Figure 2

Expression of cyclin D 1 and phospho-pRb (Ser780) in HIV-1-infected podocytes. (A) Northern and western blot detection of cyclin D1 expression in wild-type, control-infected, and HIV-1-infected podocytes during subconfluent (SC) growth at 33°C and during 14 days of differentiation of confluent podocytes at 37°C shows that neither the cyclin D1 transcript nor the elevated cyclin D1 protein in HIV-1-infected podocytes (full-length, singly-spliced, and multiply spliced HIV-1 transcripts) are significantly down-regulated by podocyte cell-cell contact or by podocyte differentiation (synaptopodin transcript) when compared to wild-type or control-infected (one transcript from LTR sequences) podocytes. (B) Immunodetection of phospho-pRb (Ser780) on several phosphorylated forms (arrows) of pRb in the asynchronously proliferating, HIV-1-infected podocytes that is absent in the quiescent, control-infected podocytes after 14 days of differentiation at 37°C. (C) Abundant foci formation in confluent HIV-1-infected podocytes but not in confluent control-infected podocytes due to the ongoing proliferation of HIV-1-infected podocytes after cell-cell contact. Blots are representative of two independent experiments.

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BMC Microbiology

ISSN: 1471-2180

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