Table 3 Summary of cPCR assay.
From: Diversity and abundance of bacteria in an underground oil-storage cavity
cPCR type | Primer used | Annealing Tm (°C) | Length of DNA fragment (bp) | Specificity | ||
|---|---|---|---|---|---|---|
| Â | Â | Â | Taget | Competitor | Sequence type | Phylogenetic group |
AB | U515f and AB1173r | 60 | 658 | 431 | KU21, KU25 | Acetobacterium |
AZ | B341f and AZ665r | 55 | 324 | 437 | KU34 | Parts of Azoarcus and Zoogloea |
BC | B341f and U533r | 55 | 170–210 | 336 | All | Bacteria |
DB | B968f and DB1286r | 55 | 318 | 437 | KB14 | Part of Desulfobacula |
DT | U515f and DT1047r | 60 | 526 | 340 | KB13, KB32, KU26 | Part of Desulfotomaculum |
DV | DV387f and DV701r | 55 | 314 | 415 | KB1, KB33, KB38 | Desulfovibrio |
EP | B341f and EP710r | 55 | 369 | 465 | KB2, KB8 | Cluster-1 bacteria |