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Table 1 Primers and probes used in this study.

From: Diversity and abundance of bacteria in an underground oil-storage cavity

Primer and probe Sequence Position (5' to 3')a Specificity Reference
EUB338 5'-GCTGCCTCCCGTAGGAGT-3' 355 to 338 Bacteria [9]
ARCH915 5'-GTGCTCCCCCGCCAATTCCT-3' 934 to 915 Archaea [10]
I-341f 5'-CCTACGGGIGGCIGCA-3' 341 to 356 Bacteria [8]
U515f 5'-GTGYCAGCMGCCGCGGTAA-3' 515 to 533 Universal [7]
U1492r 5'-GGTTACCTTGTTACGACTT-3' 1510 to 1492 Universal [7]
B341f 5'-CCTACGGGAGGCAGCAG-3' 341 to 357 Bacteria [11]
U533r 5'-TTACCGCGGCKGCTGRCAC-3' 533 to 515 Bacteria [11]
B968f 5'-AACGCGAAGAACCTTAC-3' 968 to 984 Bacteria [12]
DV387f 5'-CCTGACGCAGCGACG-3' 387 to 401 δ-Proteobacteria [13]
DV701r 5'-ACGGATTTCACTCCTACACC-3' 701 to 682 Desulfovibrio [14]
EP710r 5'-CAGTATCATCCCAGCAGA-3' 710 to 693 Cluster-1 bacteria [1]
DT1041r 5'-CACCCGAIGGTGAAAACGTAC-3' 1041 to 1021 Part of Desulfotomaculum This study
AB1173r 5'-GTGTTATCCACGGCAGTCTGCT-3' 1173 to 1152 Acetobacterium This study
AZ665r 5'-CTGCCGTACTCTAGTCATAC-3' 665 to 646 Parts of Azoarcus and Zoogloea This studyb
DB1286r 5'-AGGATTGGCTCCCCCTCACA-3' 1286 to 1267 Part of Desulfobacula This study
T7W 5'-TAATACGACTCACTATAGGGC-3'   pGEM-T vector [1]
SP6W 5'-ATTTAGGTGACACTATAGAATACTC-3'   pGEM-T vector [1]
  1. aCorresponding to the numbering in the sequence of the 16S rRNA gene of E. coli. bThis primer is a modified form of probe ZRA [38].