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Table 2 Overview of the different ITS2-fragment lengths observed and of the number of strains identified by means of ITS2-PCR.

From: Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region

Species Number of strains tested (number used for calculation of the average length) ITS2-fragment length (bp), calculated by capillary electrophoresis Previously published lengths (bp)*[9] Fragment lengths (bp) derived from Genbank sequences Genbank accession number of sequences used
   Average Range Standard deviation    
Blastoschizomyces capitatum 1 250.7    248 251 AF455443
Candida albicans 116 (19) 281.6 281.0 – 282.6 0.37 279 284 AF217609
C. dubliniensis 1 286.3     289 AF218993
C. glabrata 94 (12) 362.6 362.4 – 362.8 0.15 360 365 AF218994
C. guilliermondii 8 323.2 322.7 – 324.2 0.46 321 325 AF218996
C. humicola 1 295.8      
C. kefyr 7 375.5 375.4 – 375.8 0.17 372 378 AF218997
C. krusei 10 (2) 285.3 285.2 – 285.4 0.13 282 294 L47113
C. lipolytica 3 (2) 180.6 179.9 – 181.1 0.62   190 AF218983
C. lusitaniae 9 (4) 200.3 200.2 – 200.4 0.10 199 204 AF218970
C. norvegensis 3 (2) 267.1 266.6 – 267.1 0.62   318 AF333096
C. parapsilosis 36 (12) 254.1 253.5 – 255.4 0.60 251 259 U10989
C. rugosa 1 215.0 214.8 – 215.1 0.10   223 AF218971
C. tropicalis 34 (28) 270.6 269.3 – 271.2 0.61 269 272 273 274 AF219000 AF219001 AF218992
Cryptococcus albidus 3 353.3 352.4 – 354.7 1.26 350 353 AF218972
C. laurentii ( environmental) 2 272.1 271.9 – 272.3 0.26   275 AF218974
C. laurentii (clinical) 1 307.9    306 310 AJ421006
C. neoformans var. neoformans 8 (4) 318.3 318.0 – 318.6 0.29 315 320 AF218975
C. neoformans var. gattii 1 320.0     321 AF196312
Dekkera bruxellensis 1 261.8     263 AF043502
Geotrichum candidum 3 (1) 193.4    192 195 AF157596
Malassezia furfur 1 494.4     500 AF246896
Saccharomyces cerevisiae 35 (25) 366.6 365.1 – 368.5 0.89 363 365 366 367 AF219005 AF219006 AF219007
Trichosporon asahii 2 301.0 300.8–301.4 0.50   304 AB018014
T. asteroides 1 298.6     304 AF444416
T. inkin 1 298.7     304 AF444420
T. mucoides 1 297.2     297 AF444423
T. ovoides 1 297.5     303 AF444439
  1. *: Values obtained by using dUTP instead of dTTP for the PCR-based amplification reaction [9].