Figure 7

Immunofluorescence of the BZLF1 protein in BJAB-B1 cells. Immunofluorescence staining was performed to show production of viral protein, indicative of EBV reactivation. Stimulated cells and approximately 50,000 experimental sample cells were subjected to a cytospin and prepared similarly to the p52 staining protocol. A secondary FITC-labelled goat-anti-mouse antibody was used because the primary antibody was unlabelled. BJAB-B1 cells were stimulated with PMA/ionomycin (1 μg/ml) for the induction of the BZLF1 protein and for a positive control (A). Presence of the BZLF1 protein was observed within the CMV superinfected BJAB-B1 cells and only at 168 hours (B)