Figure 5

BZLF1 Transcript Expression in BJAB-B1 Cells. BZLF1 transcript was chemically induced with PMA/ionomycin (1 μg/ml) as a positive control for EBV reactivation. Qualitative real-time PCR was performed to show positive expression of the BZLF1 transcript. Time points were taken at 24 hours (C2), 48 hours (C9), and 72 hours (E2). E6 represents unstimulated cells and also served as a negative control (A). Real-time PCR amplification of the BZLF1 transcript was performed using 5 μl of cDNA from the RT reactions among BJAB-B1 cells superinfected with CMV. Time points are at the given designations A8 (24 hours), A9 (48 hours), A10 (72 hours), A11 (96 hours), and A12 (168 hours) (B). Agarose (2%) gel electrophoresis of the real-time PCR samples was performed in order to show specificity of the PCR reactions (C).