Real-time PCR of the CMV MIE transcript in P3HR-1 cells. In all graphs, the axes are relative fluorescence units (RFUs) versus cycle number. CMV DNA copy standards were run in order to establish a standard curve for quantitative analysis. A1 (500,000 copies), A2 (5,000 copies), A3 (50 copies), and A4 (negative control) (data not shown). cDNA from the P3HR-1 cells were treated similarly to the BJAB-B1 cells. Shown are P3HR-1 cells superinfected with CMV (A) and cells exposed to UV-irradiated CMV (B). Uninfected cells were negative for any CMV transcript (data not shown). Time points are designated in the figure. For these set of experiments, 0, 24, 48, 72, 96, 120, and 168 hours post superinfection were performed. Agarose gel analyses were not performed.