Figure 1

Real-time PCR of the CMV MIE transcript in BJAB-B1 cells. In all graphs, the axes are relative fluorescence units (RFUs) versus cycle number. Real-time PCR amplification of the CMV MIE transcript from superinfected BJAB-B1 cells was performed using 5 μl of cDNA from the RT reactions. Time points are at the given designations C7 (24 hours), C8 (48 hours), C9 (72 hours), C10 (96 hours), and C11 (168 hours) (A). Real-time PCR amplification of the CMV MIE transcript from BJAB-B1 cells incubated with UV-irradiated CMV (B). All time points were negative at the given designations G1 (24 hours), G2 (48 hours), G3 (72 hours), G4 (96 hours), and G5 (168 hours). CMV DNA copy standards were run in order to establish a standard curve for quantitative analysis. A1 (500,000 copies), A2 (5,000 copies), A3 (50 copies), and A4 (negative control) (C). Agarose (2%) gel electrophoresis of the real-time PCR samples was performed in order to show specificity of the PCR reactions. Positive control is as indicated. Shown here are the real-time PCR samples of the BJAB-B1 cells superinfected with CMV. Lanes 1–5 correlate to the time points 24, 48, 72, 96, and 168 hours (D).