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Table 2 Reference samples analysed with the two PCR assays. * The unit of the numbers is copies/μl and the numbers are the mean of two measurements from two different runs. # The dilution series is serial and four-fold. Dilution no. 1 has the highest concentration and dilution no. 10 has the lowest concentration. Method 1 is the real-time PCR and method 2 is the conventional PCR.

From: Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae

PCR method 1 2
Detection probe set* gel
Dilution no. #   
1 (high) 76.60 +
2 7.86 +
3 2.35 +
4 0.63 +
5 0.05 +
6 0.00 -
7 0.00 -
8 0.00 -
9 0.00 -
10 (low) 0.00 -
11 (NK) 0.00 -