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Table 1 Sample volumes used in the two PCR methods

From: Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae

PCR method

Method 1

Method 2

Sample type

  

Standard samples

2 μl

-

Reference samples

6.8 μl

10 μl

Clinical samples

2 μl

10 μl

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BMC Microbiology

ISSN: 1471-2180

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