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Table 1 Two hybrid analysis of alanine site-directed mutants of LcrG (residues 7–40) with LcrV.

From: Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface

Mutation

Filter-lift Reaction

β-galactosidase activitya

wildtype

+

68.05 ± 2.96

LcrG aloneb

-

6.71 ± 0.51

T12A

+

51.55 ± 5.15

L13A

+

50.80 ± 5.13

K14A

+

58.60 ± 2.57

E17A

+

54.85 ± 6.19

L18A

+

59.55 ± 0.95

I20A

+

51.85 ± 3.75

D22A

+

42.65 ± 0.85

S23A

+

66.73 ± 1.80

D24A

+

45.93 ± 3.91

R26A

+

43.27 ± 2.67

K28A

+

47.87 ± 3.43

L29A

+

47.00 ± 2.99

L30A

+

47.90 ± 3.30

E32A

+

65.25 ± 4.85

M33A

+

48.40 ± 3.00

C34A

+

46.73 ± 2.00

I37A

+

52.27 ± 2.23

  1. aβ-galactosidase activity is expressed as Miller Units ± standard error of the mean. bThis value is derived from yeast expressing LcrG and an empty vector control.
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BMC Microbiology

ISSN: 1471-2180

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