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Figure 2 | BMC Microbiology

Figure 2

From: Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface

Figure 2

Pairwise BLAST alignment of LcrG and PcrG. Amino acid sequence alignment of LcrG from Y. pestis (NCBI RefSeq, NP_395166; http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=16082720&dopt=GenPept) and PcrG from P. aeruginosa (NCBI RefSeq, NP_250396; http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=15596902&dopt=GenPept). Overall the two proteins are 43% identical and 56% similar. The sequences were aligned using the BLAST algorithm as implemented for pairwise alignments [37]. The region highlighted in yellow (amino acids 7–40) is the smallest region identified that interacts with LcrV. The region in blue (amino acids 39–53) corresponds to a previously described deletion that eliminated secretion blocking activity [5]. The amino acids identified with red asterisks are residues identified as participating in the LcrG-LcrV interaction (A16, S23, and L30). The residue marked with the blue asterisk (F48) is required for secretion blocking activity of LcrG.

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