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Figure 1 | BMC Microbiology

Figure 1

From: Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface

Figure 1

Schematic representation of LcrG truncations fused to the GAL4 activation domain of plasmid pACT2. All constructs were cotransformed with full-length LcrV cloned into cyh2-deleted pAS2-1 to produce a GAL4-DNA binding domain-LcrV chimera in S. cerevisiae strain Y187. The ability of each truncation to bind LcrV was determined qualitatively by X-gal hydrolysis from filter-lift assays and quantitatively by the level of β-galactosidase activity expressed as Miller units in yeast liquid cultures. Final β-galactosidase activities are calculated from the average of at least four independent measurements.

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