Figure 1

DNA quality analysis. A – Agarose gel electrophoresis of DNA sample purified by the cellophane disk miniprep method. Lane 1 (1 Kb ladder), lane 2–3, genomic DNA from Trichoderma reesei transformants 1 and 2, respectively. B – Amplified hph fragment from DNAs prepared by the cellophane-base method. Agarose gel electrophoresis of PCR samples. Lane 1 (1 Kb ladder), lane 2–3, 750 pb PCR products from transformant 1 and 2, respectively. C – Detection of hph gene in DNA prepared from transformants of Trichoderma reesei. The DNA obtained by the cellophane method was Bam HI and Eco RI restricted and subjected to Southern Blot, using an α-³²PdATP labeled fragment of the hph gene as a probe. Lane 1 and 4 (1 Kb DNA ladder, probed against itself), lane 2 and 5, restricted DNA from hph Trichoderma reesei transformant 1 and 2, respectively; 3 and 6, DNA from untransformed Trichoderma reesei (control).