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Table 1 Strains, plasmids, and phages.

From: Oxygen and nitrate-dependent regulation of dmsABC operon expression in Escherichia coli: sites for Fnr and NarL protein interactions

Strains, plasmids and phages

Parent

Genotype or Phenotype

Source

Strains

   

MC4100

 

F-araD139 (argF-lac) U169 rpsL150 relA1 flb5301 deoC1 ptsF25 rbsR

[38]

PC2

MC4100

fnr

[4]

Plasmids

   

pPC25

 

pDMS

[4]

pJA250

 

dmsA-lacZ TTGATaccgAACAA

this study

pJA303

 

dmsA-lacZ TTGATaccgAT CAA

this study

pJA450

 

dmsA-lacZ TTGT TaccgAT CAA

this study

pJA257

 

dmsA-lacZ TTAG TaccgAACAA

this study

pJA304

 

dmsA-lacZ ----Δ----accgAT CAA

this study

pJA448

 

dmsA-lacZ TTGATaccgAACAAA

this study

pJA449

 

dmsA-lacZ TTGATaccgAACAAC

this study

pRS415

 

lacZ lacY + lacA +

[35]

pRS1274

 

lacZ lacY + lacA +

[35]

pBluescript

  

Stratagene

pBSDMS4

pBluescript

dmsA'

this study

pGEM-11Zf

  

Promega

pSB10

pGEM-11Zf

dmsA -127 to -13

this study

Phage

   

M13mp19-100

M13mp19

dmsA'

laboratory stock

λRS45

 

lacZ

[35]

λPC25

λRS45

dmsA-lacZ lacY + lacA +

[4]