Figure 3

DNase I and hydroxyl radical footprint analyses of the dmsA non-coding strand by NarL and NarL-phosphate. The closed boxes denote the hydroxyl radical protected regions whereas the open box indicates the DNase I protected region. The asterisks note positions with increased sensitivity to DNase I cleavage when NarL-phosphate is bound to the DNA. Numbering of the DNA is relative to the start of dmsA transcription. The amount of NarL used in each lane is indicated above each lane. Lane G contains the Maxam-Gilbert sequencing reaction. Lanes 1 and 5–8 designate the phosphorylated NarL protein.