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Table 1 hemoFISH Gram positive and Gram negative panels performances in identifying Gram-negative and Gram-positive in comparison with Vitek 2 system

From: Beacon-based (bbFISH®) technology for rapid pathogens identification in blood cultures

Panel Species Strains identified using Vitek 2 system Strains identified using bbFISH bbFISH global percentage of identification bbFISH (%) Strains misidentified by bbFISH Strains not identified by bbFISH
hemoFISH Gram positive S.epidermidis and other CoNS # 131 130& 221/239 (92.5%)   1
S.aureus 16 16   
Streptococcus spp 27 27^   
S.pneumoniae 5 3^   2
S.pyogenes 1 1   
E.faecalis 19 19   
E.faecium 22 22   
E.gallinarum 1 0  
E.raffinosus 2 0   2
M.luteus 4   2& 2
M.lylae 4 0   4
Corynebacterium spp. 2 0   2
Bacillus spp. 2 0   2
C.perfringens 3 3   
  C.albicans 1    
hemoFISH Gram negative E.coli 59 59 143/153 (93.5%)   
K.pneumoniae 26 24 2*  
K.oxytoca 5 5*   
E.aerogenes 4 4*   
E.cloacae 5 5*   
P.mirabilis 5 4* 1*  
P.vulgaris 2 2   
S.enterica 4 4a   
S.marcescens 5 5   
P.multocida 1   1  
P.aeruginosa 14 14   
A.baumannii 11 11b   
A.lwoflii 1 1b   
S.maltophilia 4 4   
B.cepacia 2 0   2
A.veronii 1   
H.influenzae 1 1   
R.radiobacter 1 0   1
B.fragilis 2 0   2
Total 393 364   8 21
  1. CoNS# = Staphylococcus coagulase negative, namely: S.capitis, S.hominis, S.haemolyticus, S.warneri, S.auricolaris, S.saccarolyticus, S.cohnii; & = Staphylococcus spp; ^ = Streptococcus spp; ° misidentified as E.faecium; § aspecific signal on green channel (eubacterial probe); * = Enterobacteriaceae spp; a = Salmonella spp; b Acinetobacter spp; c Streptococcus spp, namely (S.bovis, S.oralis, S.gallolyticus and S.gordonii).