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Table 1 hemoFISH Gram positive and Gram negative panels performances in identifying Gram-negative and Gram-positive in comparison with Vitek 2 system

From: Beacon-based (bbFISH®) technology for rapid pathogens identification in blood cultures

Panel

Species

Strains identified using Vitek 2 system

Strains identified using bbFISH

bbFISH global percentage of identification bbFISH (%)

Strains misidentified by bbFISH

Strains not identified by bbFISH

hemoFISH Gram positive

S.epidermidis and other CoNS #

131

130&

221/239 (92.5%)

 

1

S.aureus

16

16

  

Streptococcus spp

27

27^

  

S.pneumoniae

5

3^

 

2

S.pyogenes

1

1

  

E.faecalis

19

19

  

E.faecium

22

22

  

E.gallinarum

1

0

 

E.raffinosus

2

0

 

2

M.luteus

4

 

2&

2

M.lylae

4

0

 

4

Corynebacterium spp.

2

0

 

2

Bacillus spp.

2

0

 

2

C.perfringens

3

3

  
 

C.albicans

1

   

hemoFISH Gram negative

E.coli

59

59

143/153 (93.5%)

  

K.pneumoniae

26

24

2*

 

K.oxytoca

5

5*

  

E.aerogenes

4

4*

  

E.cloacae

5

5*

  

P.mirabilis

5

4*

1*

 

P.vulgaris

2

2

  

S.enterica

4

4a

  

S.marcescens

5

5

  

P.multocida

1

 

1

 

P.aeruginosa

14

14

  

A.baumannii

11

11b

  

A.lwoflii

1

1b

  

S.maltophilia

4

4

  

B.cepacia

2

0

 

2

A.veronii

1

 

 

H.influenzae

1

1

  

R.radiobacter

1

0

 

1

B.fragilis

2

0

 

2

Total

393

364

 

8

21

  1. CoNS# = Staphylococcus coagulase negative, namely: S.capitis, S.hominis, S.haemolyticus, S.warneri, S.auricolaris, S.saccarolyticus, S.cohnii; & = Staphylococcus spp; ^ = Streptococcus spp; ° misidentified as E.faecium; § aspecific signal on green channel (eubacterial probe); * = Enterobacteriaceae spp; a = Salmonella spp; b Acinetobacter spp; c Streptococcus spp, namely (S.bovis, S.oralis, S.gallolyticus and S.gordonii).