Validation of the MNGC assay (10 h post-infection). (A) Same as Figure 3A, except that macrophages were fixed at 10 h post-infection for different strains of Bp. Scale bar: 90 μm. (B) HCI quantification of several cellular features of MNGC formation and (C) bacterial features from images acquired as described in Figure 3A. In B and C means +/- SD are shown of 6 replicates per plate, 3 plates run on independent days (n = 18). For each replicate well >1000 nuclei were analyzed. **** p <0.0001; *** p < 0.001.