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Table 2 Bacteriological characterization, virulence gene profile and cdt subtype of CDT-producing Escherichia coli isolated from cattle and swine in Japan

From: Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan

Host CDT subtype Serotype PG1 n= CDT-III and -V subtyping Virulence gene
          DEC8 Adhesin9 NTEC10
      cdt-IIIi 2 cdt-Vi 3 cdt-IIIABC 4 cdt-V up5/down6 stx1 stx2 subAB eaeA astA saa lpfAO113 ehaA iha cnf2
Cattle CDT-I O112ac:H20 B1 1 ND7 ND ND ND/ND - - - - - - + + - -
   OUT:H26 D 1 ND ND ND ND/ND - - - - - - - + - -
  CDT-IV O169:H10 B2 1 ND ND ND ND/ND - - - - + - - - - -
  CDT-III O2:HUT B2 3 + - + -/- - - - - - - - - - +
   O2:NM B2 1 + - + -/- - - - - - - - - - +
   O7:H6 B1 1 + - + -/- - - - - - - + + - +
   O88:H2 B1 1 + - + -/- - - - - - - + + - +
   O88:H4 B1 1 + - + -/- - - - - + - + + - +
   O88:H6 B1 1 + - + -/- - - - - - - + + - +
   OUT:H1 B1 1 + - + -/- - - - - - - + + - +
   OUT:H21 B1 11 + - + -/- - - - - + - + + - +
   OUT:H45 D 1 + - + -/- - - - - - - - + - +
   OUT:HUT B1 1 + - + -/- - - - - - - + + - +
   OUT:NM B1 3 + - + -/- - - - - - - + + - +
  CDT-V O2:H10 B2 1 - + - +/+ - - - - - - - - - -
   O8:HUT B1 1 - + - +/+ - - - - - - + - - +
   O22:H8 B1 5 - + - +/+ + (412/513) + (4/5) - - + (1/5) + + + + -
   O22:HUT B1 2 - + - +/+ + + - - - + + + + -
   O113:H21 B1 3 - + - +/+ - + + - - + + + + -
   O113:NM B1 2 - + - +/+ - + + - - + + + + -
   O118:NM B1 1 - + - +/+ - - - - + - + + - -
   O154:H34 B1 1 - + - +/+ - - - - - - + + + -
   O156:HUT B1 3 - + - +/+ - - - +11 - - + + - -
   O163:HUT B1 1 - + - +/+ - - - - + - + + - -
   OUT:H1 B1 1 - + - +/+ - - - - - - + - - +
   OUT:H19 B1 2 - + - +/+ - - - - + - + + - -
   OUT:H2 B1 5 - + - +/+ - - - - - - + - - +
   OUT:H21 B1 1 - + - +/+ - - - - - - + + - -
   OUT:H25 B1 1 - + - +/+ + + + - - + + + + -
   OUT:H48* D 2 - + - -/- - - - - + - - + - -
   OUT:H6 B1 2 - + - +/+ + + - - - + + + + -
   OUT:H8 B1 5 - + - +/+ + + - - - + + + + -
   OUT:HUT B1 7 - + - +/+ + (2/7) + (2/7) - - + (4/7) + (2/7) + + + (2/7) -
   OUT:NM B1 6 - + - +/+ - + (1/6) + (1/6) - + (5/6) + (1/6) + + + (1/6) -
  CDT-III and V O2:HUT* B2 1 + + - -/- - - - - + - - - - -
Swine CDT-V O98:H10* B1 5 - + - -/+ - - - - + - + + - -
   OUT:HUT* B1 1 - + - -/+ - - - - + - + + - -
  CDT-II O84:NM14 D 1 ND ND ND ND/ND - - - + - - - - - -
  1. bfp, EAF, elt, est, aggR, invE genes for DEC, cnf1 for NTEC, and efa1 for adhesin were negative in all strains tested.
  2. *Not properly differentiated by previous type-specific PCR assays, 1phylogenetic group, 2PCR result by CdtIII/VB-F and CdtIIIC-R primers, 3PCR result by CdtIII/VB-F and CdtVC-R primers, 4PCR result by Cdt-IIIAf and Cdt-IIIACr primers 5PCR result by P2-A2 and cdtA-F primers, 6PCR result by cdtC-F and P2-C3 primers, 7not done, 8genes for DEC, 9genes for Adhesin, 10gene for NTEC, 11eae-θ/γ2, 12No. of positive strains, 13No. of tested strains, 14identified as Escherichia albertii.