Table 1 Differentially expressed protein spots between X. citri and hrpB − strains statically cultured in XVM2 with a change abundance of minimum 1.5 fold and p value of < 0.05 (ANOVA)
X. citri gene no. | Protein name | MOWSE score | Accession no. | Predicted MW/pI | Observed MW/pI | Peptide match/coverage | Fold change compared to WT |
|---|---|---|---|---|---|---|---|
01 Metabolism | |||||||
01.01 Amino acid metabolism | |||||||
XAC0125 | Aspartate/tyrosine/aromatic aminotransferase | 350 | Q8PR41_XANAC | 43.3/5.72 | 49.0/4.8 | 19/38% | 1.9 |
XAC4034 | Shikimate 5-dehydrogenase | 297 | AROE_XANAC | 29.9/4.93 | 30.0/5.9 | 19/17% | 2.4 |
XAC2717 | Tryptophan synthase subunit b | 31 | TRPB_XANAC | 43.3/5.88 | 53.0/4.6 | 2/4% | 7.5 |
XAC3709 | Tryptophan repressor binding protein | 48 | Q8PGA8_XANAC | 20.0/6.40 | 10.0/4.4 | 3/17% | −1.6 |
01.02 Nitrogen, sulfur and selenium metabolism | |||||||
XAC0554 | NAD(PH) nitroreductase | 208 | Y554_XANAC | 21.0/5.83 | 18.0/4.7 | 14/38% | 4.6 |
01.03 Nucleotide/nucleoside/nucleobase metabolism | |||||||
XAC1716 | CTP-synthase | 125 | PYRG_XANAC | 61.7/5.91 | 67.0/4.5 | 14/21% | 3.5 |
01.05 C-compounds and carbohydrate metabolism | |||||||
XAC2077 | Succinate dehydrogenase flavoprotein subunit | 192 | Q8PKT5_XANAC | 65.8/5.89 | 66.0/4.6 | 20/25% | 2.2 |
XAC1006 | Malate dehydrogenase | 1054 | MDH_XANAC | 34.9/5.37 | 45.0/5.4 | 55/50% | −1.8 |
XAC3579 | Phosphohexose mutases (XanA) | 98 | Q8PGN7_XANAC | 49.1/5.29 | 54.0/5.6 | 7/10% | 1.7 |
XAC3585 | DTP-glucose 4,6-dehydratase | 235 | Q8PGN1_XANAC | 38.6/5.86 | 48.0/4.7 | 12/17% | 2.1 |
XAC0612 | Cellulase | 245 | Q8PPS3_XANAC | 51.6/5.76 | 57.0/4.9 | 23/32% | 2.6 |
XAC3225 | Transglycosylase | 178 | Q8PHM6_XANAC | 46.2/5.89 | 53.0/4.8 | 14/22% | −1.6 |
01.06 Lipid, fatty acid and isoprenoid metabolism | |||||||
XAC3300 | Putative esterase precursor (EstA) | 96 | Q8PHF7_XANAC | 35.9/6.03 | 62.0/6.2 | 3/4% | −3.1 |
XAC1484 | Short chain dehydrogenase precursor | 104 | Q8PME5_XANAC | 26.0/5.97 | 30.0/4.4 | 5/9% | 2.2 |
01.06.02 Membrane lipid metabolism | |||||||
XAC0019 | Outer membrane protein (FadL) | 167 | Q8PRE4_XANAC | 47.3/5.18 | 46.0/6.1 | 8/10% | −10.0 |
XAC0019 | Outer membrane protein (FadL) | 79 | Q8PRE4_XANAC | 47.3/5.18 | 35.0/6.0 | 7/13% | −6.2 |
01.20 Secondary metabolism | |||||||
XAC4109 | Coproporphyrinogen III oxidase | 46 | HEM6_XANAC | 34.6/5.81 | 37.0/4.9 | 8/19% | 1.5 |
02 Energy | |||||||
02.01 Glycolysis and gluconeogenesis | |||||||
XAC1719 | Enolase | 90 | ENO_XANAC | 46.0/4.93 | 55.0/5.9 | 7/13% | 1.7 |
XAC3352 | Glyceraldehyde-3-phosphate dehydrogenase | 267 | Q8PHA7_XANAC | 36.2/6.03 | 46.0/4.4 | 24/28% | 2.6 |
XAC2292 | UTP-glucose-1-phosphate uridylyltransferase (GalU) | 92 | Q8PK83_XANAC | 32.3/5.45 | 38.0/5.3 | 13/30% | 4.2 |
02.07 Pentose phosphate pathway | |||||||
XAC3372 | Transketolase 1 | 85 | Q8PH87_XANAC | 72.7/5.64 | 69.0/4.9 | 5/7% | 5.0 |
02.11 Electron transport and membrane-associated energy conservation | |||||||
XAC3587 | Electron transfer flavoprotein a subunit | 50 | Q8PGM9_XANAC | 31.8/4.90 | 34.0/5.5 | 6/14% | 2.3 |
10 Cell cycle and DNA processing | |||||||
10.03 Cell cycle | |||||||
XAC1224 | Cell division topological specificity factor (MinE) | 33 | MINE_XANAC | 9.6/5.37 | 12.0/4.9 | 1/14% | 2.7 |
10.03.03 Cytokinesis/septum formation and hydrolysis | |||||||
XAC1225 | Septum site-determining protein (MinD) | 143 | Q8PN48_XANAC | 28.9/5.32 | 34.0/5.6 | 19/26% | 2.3 |
11 Transcription | |||||||
XAC0996 | DNA-directed RNA polymerase subunit a | 104 | RPOA_XANAC | 36.3/5.58 | 33.0/5.0 | 5/7% | −4.3 |
XAC0966 | DNA-directed RNA polymerase subunit b | 150 | RPOC_XANAC | 155.7/7.82 | 35.0/4.6 | 16/8% | −3.3 |
14 Protein fate (folding, modification and destination) | |||||||
14.01 Protein folding and stabilization | |||||||
XAC0542 | 60 kDa chaperonin (GroEL) | 199 | CH60_XANAC | 57.1/5.05 | 41.0/5.5 | 15/27% | −11.2 |
16 Protein with binding function or cofactor requirement | |||||||
XAC2726 | Adenine-specific methylase (Dam methylase) | 84 | Q8PJ19_XANAC | 33.9/4.70 | 41.0/6.2 | 10/12% | −1.9 |
XAC1362 | GTN reductase | 44 | Q8PMR4_XANAC | 39.4/5.37 | 50.0/5.3 | 7/10% | 2.3 |
XAC3664 | OmpW family outer membrane protein precursor | 226 | Q8PN48_XANAC | 23.8/4.97 | 28.0/6.2 | 12/13% | 2.3 |
30 Cellular communication/Signal transduction mechanism | |||||||
XAC0291 | Oar protein ( TonB-dependent transporter) | 50 | Q8PQN2_XANAC | 107.9/5.29 | 108.0/5.7 | 2/1% | 4.3 |
XAC2672 | Oar protein ( TonB-dependent transporter) | 280 | Q8PJ70_XANAC | 117.4/5.10 | 90.0/5.9 | 19/18% | 2.4 |
XAC4273 | TonB-dependent transporter | 100 | Q8PJL0_XANAC | 109.2/5.14 | 90.0/5.6 | 3/3% | 2.8 |
XAC1143 | TonB-dependent transporter | 576 | Q8PND0_XANAC | 87.7/5.21 | 70.0/6.1 | 30/33% | 1.7 |
XAC3050 | TonB-dependent transporter | 596 | Q8PI48_XANAC | 105.8/4.76 | 64.0/6.2 | 30/16% | −3.0 |
XAC3444 | TonB-dependent transporter | 1280 | Q8PH16_XANAC | 103.2/4.79 | 90.0/6.3 | 84/37% | 3.9 |
XAC3168* | TonB-dependent transporter | 98 | Q8PHT1_XANAC | 87.3/5.20 | 59.0/6.0 | 3/3% | −3.1 |
XAC3166* | TonB-dependent transporter | 410 | Q8PHT3_XANAC | 84.5/4.95 | 69.0/6.1 | 22/18% | −2.9 |
XAC3489 | TonB-dependent transporter | 685 | Q8PGX3_XANAC | 88.9/4.93 | 69.0/5.9 | 40/24% | −1.7 |
XAC1413 | Outer membrane protein assembly factor BamA | 135 | Q8PML3_XANAC | 87.6/5.53 | 88.0/5.4 | 13/15% | 2.8 |
32 Cell rescue, defense and virulence | |||||||
XAC2504* | Regulator of pathogenicity factors (RpfN) | 271 | Q8PJM6_XANAC | 41.3/5.98 | 49.0/4.4 | 21/16% | −4.8 |
XAC0907 | Alkyl hydroperoxide reductase subunit C | 240 | O06464_XANAC | 20.6/6.15 | 20.0/4.2 | 28/61% | 1.3 |
32.07 Cellular detoxification | |||||||
XAC1474 | Glutathione transferase | 39 | Q8PMF5_XANAC | 23.9/6.06 | 22.0/4.7 | 4/8% | 1.7 |
34 Interaction with the environment | |||||||
34.01 Homeostasis | |||||||
XAC1149 | Bacterioferritin | 100 | Q8PNC4_XANAC | 21.2/4.71 | 20.0/6.3 | 6/20% | 2.1 |
XAC0493 | Bacterioferritin | 152 | Q8PQ38_XANAC | 18.3/4.80 | 12.0/6.5 | 19/43% | 2.5 |
XAC1533 | Dihydrolipoamide dehydrogenase | 336 | Q8PM99_XANAC | 50.5/5.80 | 59.0/4.6 | 34/47% | 4.0 |
42 Biogenesis of cellular components | |||||||
XAC1230 | Putative membrane protein | 71 | Q8PN43_XANAC | 43.1/6.88 | 24.0/4.4 | 4/11% | −3.5 |
99 Unclassified proteins | |||||||
XAC1262 | Protein of unknown function (Aminopeptidase) | 121 | Q8PN12_XANAC | 63.4/5.85 | 68.0/4.6 | 13/15% | 5.3 |
XAC1344 | Protein of unknown function (CcmA) | 67 | Q8PMT2_XANAC | 18.7/5.45 | 23.0/5.7 | 4/18% | −1.7 |