Figure 5
Expression analyses of genes for AF and kojic acid production and sugar utilization. (A) qRT-PCR analyses of expression of 9 AF biosynthetic genes (aflR, aflS, aflC, aflD, aflM, aflP, aflO, aflU, and nadA) and 3 sugar utilization genes (hxtA, glcA and sugR) in mycelia grown with or without 40 mg/mL D-glucal for 3 d, The relative expression levels were quantified through comparison with the expression level of β-tubulin. Data are presented as means ± S.D. (n = 3). (B) Expression of 3 kojic acid biosynthetic genes (kojA, kojR, kojT) by qRT-PCR in mycelia grown with or without 40 mg/mL D-glucal for 3 d. The relative expression levels were quantified through comparison with the expression level of β-tubulin. Data are presented as means ± S.D. (n = 3).