Mycelia grown in media with or without D-glucal showed significant differences in the accumulation of various metabolites. PLS analyses were performed using SIMCA-P V12.0. (A) Loadings plot obtained from PLS analyses of the entire GC-TOF MS dataset. (B) PLS scores plot of different metabolites in mycelia cultured for 5 d in GMS media with (▲1) or without (▲2) 40 mg/mL D-glucal, showing clear differences between these two sample groups. Nine replicates for each treatment.