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Table 5 Bacterial strains, plasmids and primers used in this study

From: The requirement for the LysR-type regulator PtrA for Pseudomonas chlororaphis PA23 biocontrol revealed through proteomic and phenotypic analysis

Strain/plasmid/primer Relevant genotype or phenotype Source or reference
P. chlororaphis PA23 Phz+RifR wild type (soybean plant isolate) [1]
PA23-443 Phz RifR ptrA::Tn5- OT182 genomic fusion This study
E. coli   
DH5α supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Gibco
SM10 Mobilizing strain; RP4 tra genes integrated in chromosome; KmR TcR [33]
Plasmids   
pOTI82 pSUP102(GM)::Tn5-OT182 CmR GmR AmpR TcR [34]
pOT182-443 (Xho I) pOT182 containing ptrA::Tn5-OT182 genomic fusion This study
pCR2.1TOPO Cloning vector for PCR products Invitrogen
pUCP22 Broad-host-range vector; IncP OriT, AmpR GmR [35]
pUCP22-ptrA pUCP22 containing ptrA from P. chlororaphis PA23 This study
Primers   
ptrA-F 5′-gggaaccggcttatagcca-3′ This study
ptrA-R 5′-atccagttgctggagcgtatt-3′ This study
TNP5-FORWARD 5′-accatttcaacggggtctcac-3′ [4]
TNP5-REVERSE 5′-tgactccatgtgacctccta-3′ [4]
Tn5-ON82 5′-gatcctggaaaacgggaaagg-3′ [4]
Tn5-OT182 right 5′-atgttaggaggtcacatg-3′ [4]