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Table 5 Bacterial strains, plasmids and primers used in this study

From: The requirement for the LysR-type regulator PtrA for Pseudomonas chlororaphis PA23 biocontrol revealed through proteomic and phenotypic analysis

Strain/plasmid/primer

Relevant genotype or phenotype

Source or reference

P. chlororaphis PA23

Phz+RifR wild type (soybean plant isolate)

[1]

PA23-443

Phz RifR ptrA::Tn5- OT182 genomic fusion

This study

E. coli

  

DH5α

supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Gibco

SM10

Mobilizing strain; RP4 tra genes integrated in chromosome; KmR TcR

[33]

Plasmids

  

pOTI82

pSUP102(GM)::Tn5-OT182 CmR GmR AmpR TcR

[34]

pOT182-443 (Xho I)

pOT182 containing ptrA::Tn5-OT182 genomic fusion

This study

pCR2.1TOPO

Cloning vector for PCR products

Invitrogen

pUCP22

Broad-host-range vector; IncP OriT, AmpR GmR

[35]

pUCP22-ptrA

pUCP22 containing ptrA from P. chlororaphis PA23

This study

Primers

  

ptrA-F

5′-gggaaccggcttatagcca-3′

This study

ptrA-R

5′-atccagttgctggagcgtatt-3′

This study

TNP5-FORWARD

5′-accatttcaacggggtctcac-3′

[4]

TNP5-REVERSE

5′-tgactccatgtgacctccta-3′

[4]

Tn5-ON82

5′-gatcctggaaaacgggaaagg-3′

[4]

Tn5-OT182 right

5′-atgttaggaggtcacatg-3′

[4]