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Table 3 Strains and plasmids

From: Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei

Strain/plasmid Description Reference
B. pseudomallei   
DD503 Parental strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive (derived from clinical isolate 1026b) [61]
bpaC KO Isogenic bpaC mutant strain of DD503; polymixin B resistant, zeocin resistant, kanamycin sensitive This study
B. mallei   
ATCC 23344 Wild-type strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive [75]
bpaC KO Isogenic bpaC mutant strain of ATCC 23344; polymixin B resistant, zeocin resistant, kanamycin sensitive This study
E. coli   
EPI300 Cloning strain epicentre® Illumina®
TUNER™ Expression strain for purification of His-tagged BpaC EMD Millipore
S17 Strain used for conjugational transfer of pKASbpaC.zeo to B. pseudomallei and B. mallei [76]
pCC1™ Cloning vector, chloramphenicol resistant epicentre® Illumina®
pCCbpaC pCC1 containing the B. pseudomallei DD503 bpaC gene, chloramphenicol resistant This study
pCCbpaC.zeo pCCbpaC in which a zeocin resistance cassette was introduced near the middle of the bpaC ORF; chloramphenicol and zeocin resistant This study
pCC1.3 pCC1-based plasmid control, does not confer adherence to human epithelial cells; chloramphenicol resistant [77]
pKAS46 Mobilizable suicide plasmid; kanamycin resistant [78]
pKASbpaC.zeo pKAS46 containing the insert from pCCbpaC.zeo This study
pEM7ZEO Source of the zeocin resistance marker Life Technologies™
pELHisBPSL1631-BMA1027 Plasmid expressing aa 392–1068 of B. pseudomallei 1026b BpaC fused to six N-terminal histidine residues, introduced in E. coli TUNER and used to purify His-tagged BpaC protein for antibody production and ELISA experiments; chloramphenicol resistant. [67]