Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei

Table 2 Median lethal dose determination of B. mallei and B. pseudomallei WT and mutant strains

Organism	Strain	Inoculating dose (CFU)	Group size	% death	LD₅₀(CFU)
B. mallei ^a	ATCC 23344 (WT)	9,100	5	100
		5,550	5	100
		910	9	78	346
		455	5	40
		91	9	11
B. mallei ^a	bpaC KO (mutant)	10,400	5	100
		5,200	6	83
		1,040	9	100	238
		520	5	40
		104	9	22
PBS (control)^a		0	5	0
B. pseudomallei ^b	DD503 (WT)	380,000	5	100
		38,000	5	100	1,202
		3,800	5	100
		380	5	0
B. pseudomallei ^b	bpaC KO (mutant)	350,000	5	100
		35,000	5	100	1,107
		3,500	5	100
		350	5	0
PBS (control)^b		0	5	0

^amice were monitored daily for clinical signs of illness/morbidity for 10 days post-inoculation.
^bmice were monitored daily for clinical signs of illness/morbidity for 6 days post-inoculation.

ISSN: 1471-2180