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Table 4 The specific primers for PCR amplification

From: Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum

Name Primer sequence
fpsssbNcoI 5′ GGA GGA C CA TGG GGA ACG GAA CGT TAA ATA AAG TCA TG 3′
fpsssbHindIII 5′ TTA AAG CTT TTA AAA AGG CAA ATC ATT TTC TAC AG 3′
pcrssbNcoI 5′ TTA CC A TGG GGC GCG GTG TTA ATA AAG TTA TCA TC 3′
pcrssbHindIII 5′ TTA AAG CTT TCA GAA CGG AAT GTC ATC GTC 3′
ptossbNcoI 5′ GGA GGA CC A TGG CAG GAA CAC TCA ATA AAG TTA TGC 3′
ptossbHindIII 5′ TTA AAG CTT TTA AAA GGG TAG ATC ATC TTC CTC 3′
pprssbNcoI 5′ GGA GGA CC A TGG CCA GTC GTG GTG TAA ATA AGG 3′
pprssbBglII 5′ TTA AGA TCT CTA GAA TGG GAT ATC ATC ATC AAA ATC 3′
dpsssbNcoI 5′ TTA CC A TGG GGA TAA ATA AGG CAA TTT TAA TTG GTA ATC TAG 3′
dpsssbHindIII 5′ TTA AAG CTT CTA GAA GGG TAC GTC GTT AC 3′
parssbNcoI 5′ GGA GGA CC A TGG GGC GCG GTG TTA ATA AAG TTA TCA TC 3′
parssbBglII 5′ TTA AGA TCT CTA GAA AGG AAT GTC ATC GTC 3′
pinssbNcoI 5′ TTA CC A TGG GGT TTA ACC GAA GCG TAA ACA AAG TAG 3′
pinssbHindIII 5′ TTA AAG CTT CTA AAA AGG AAT ATC ATC ATC GAA ATC 3′
  1. The boldface parts of the primers sequences are complementary to the nucleotide sequences of the ssb-like genes and the underlined parts are the recognition sites for restriction endonucleases.