Figure 5From: Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum Melting profiles of dsDNA and its complexes with SSB proteins. A 0.67 nmol sample of duplex DNA (44 bp) was incubated alone (1) and with 4 nmol of the Dps SSB (2), Fps SSB and Pin SSB (3), Par SSB (4), Ppr SSB(5), Pcr SSB (6), Eco SSB (7) and Pto SSB (8), in a standard buffer containing 0.1 NaCl. Absorbance changes were measured at 260 nm.Back to article page