Primer name | Sequence 5′-3′ | Purpose |
---|---|---|
pyrGN2 | CACATGCCTCATTTTGACCA | Mutant confirmation |
PyrtpsAup | ACCGTTGGAAGGTGGGATCCTATGGATCTCAGAA | Amplifies pyrG with 3' tpsA overhangs |
PyrtpsAdown | CCTTTCAGAATGAGTGTGAGCGGATAACAATTTC | |
tpsAup | CCATCTGTCTAGCTCTTCATCCCC | tpsA, upstream fragment |
tpsApyrup | GATCCATAGGATCCCACCTTCCAACGGTGTAGAGACTCC | |
tpsApyrdown | TTATCCGCTCACACTCATTCTGAAAGGTGGGGTTTTC | tpsA, downstream fragment |
tpsAdown | GCAAGATTCCCGCATCCATC | |
tpsAupN1 | CAACCCCACCAGTTCTCTCAAG | Amplification of KO-fragment |
tpsAdownN1 | AAAGGGAGTTCCAAGCAGCCTG | |
pyrtpsBup* | ATCTGCTCTGCCTGGGATCCTATGGATCTCAGAA | Amplifies pyrG with 3′ tpsB overhangs |
pyrtpsBdown | CTGCCCATCACCATGTGAGCGGATAACAATTTC | |
tpsBup* | TTGAACCCTTGAAACCGAACAC | tpsB, upstream fragment |
tpsBpyrGup* | GATCCATAGGATCCCAGGCAGAGCAGATACTTACCCGTC | |
tpsBpyrGdown | TTATCCGCTCACATGGTGATGGGCAGACGATTG | tpsB, downstream fragment |
tpsBdown | TGCTAAAGAGGGTGTGGGATTG | |
tpsBupN3 | TCCCGATTGGTAGAATCCCTAAAG | Amplification of tpsB KO-fragment |
tpsBdownN3 | CATGCGAAAATGACAGGAACATTC | |
pyrGuphind | TAAAAGCTTCTATATTGATCCTTA | pyrG, KO of tpsC |
pyrGdown | TGTGAGCGGATAACAATTTC | |
tpsCupN-2 | TGCCGAATTGACGTGCGTAGAG | Cloning of tpsC |
tpsCdownN-2 | TGGTGGTGAACCTTTCGTTGTTC | |
tpsCupN5 | CCCTCCATACTTACTCCATACATCTCG | Amplification of tpsC KO-fragment |
tpsCdownN5 | CCAGCTTGACACATCCAACATAAC | |
pyrtppAup | CCTGTCCCCGCTTCAAGAAAGGGATCCTATGGATCTCAGAA | pyrG with 3′ tppA overhangs |
pyrtppAdown | GAGTCATCAGTGCTGCTTTCTGCTGTGAGCGGATAACAATTTC | |
TppAup | TGTTGGAAGCGTCTTTCTGCC | tppA, upstream fragment |
tppApyrup | TTCTGAGATCCATAGGATCCCTTTCTTGAAGCGGGGACAGG | |
tppApyrdown | GAAATTGTTATCCGCTCACAGCAGAAAGCAGCACTGATGACTC | tppA, downstream fragment |
tppAdown | TGTCCGATTGGGGGTGATTG | |
tppAupN1 | TGAGGAGGCGTTGTCAAAAGATAG | Amplification of tppA KO-fragment |
tppAdownN1 | CGATTGGGGGTGATTGGCTTAC | |
pyrtppBup | CGGTAGGTTAGGGATCCTATGGATCTCAGAA | Amplification of A. oryzae pyrG with 3′ tppB overhangs |
pyrtppBdown | GTTTGTCTTGTGTGAGCGGATAACAATTTC | |
tppBup | ATACCAAGCAATCGCCCAAGCCAG | tppB, upstream fragment |
tppBpyrGup | TCCATAGGATCCCTAACCTACCGCCCAAAGAGAGAGC | |
tppBpyrGdown | TTGTTATCCGCTCACACAAGACAAACGATGCGGAATG | tppB, downstream fragment |
tppBdown | CGTATCCTGGACTTTCAGCACG | |
tppBupN1 | TTTTCGACCTTGGTGGGTGCTTCC | Amplification of tppB KO-fragment |
tppBdownN1 | GAGACATTGTCGGTCAGTGAGGTAG | |
pyrtppCup | TGTCCTTCAGGGATCCTATGGATCTCAGAA | pyrG with 3′ tppC overhangs |
pyrtppCdown* | CTGTTCAGCATTGTGAGCGGATAACAATTTC | |
tppCup | ATGAGGTGATAGTCGTGGACCCAG | tppC, upstream fragment |
tppCpyrup | TCCATAGGATCCCTGAAGGACAAAGACAGGCTGAAG | |
tppCpyrdown* | TTGTTATCCGCTCACAATGCTGAACAGATGATCCCCAG | tppC, downstream fragment |
tppCdown* | TCGAGGTAGAGGTTCCCTTTCG | |
tppCupN1* | CGATAGTCTTTGCGAACAGACGG | Amplification of tppC KO-fragment |
tppCdownN1* | CGAGGTAGAGGTTCCCTTTCGATG | |
tpsBupN1 | CCCTTTCCCGATTGGTAGAATC | Amplification of tpsB/tppC double mutant |