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Figure 2 | BMC Microbiology

Figure 2

From: Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens

Figure 2

M. bovis BCG clearance and mycobacterial-induced lung pathology is not influenced by an established or successive T. muris infection. (A) Viable pulmonary M. bovis BCG CFU numbers at experimental endpoint in co-infected (black) and BCG-only (clear) infected BALB/c mice infected according to experimental design as shown in Figure 1A. Data display mean ± SEM, representing 3 individual experiments of 5–6 animals per group. (B) Viable pulmonary M. bovis BCG CFU numbers at experimental endpoint in co-infected (black) and BCG-only (clear) infected BALB/c mice infected according to experimental design as shown in Figure 1B. Data display mean ± SEM, representing 3 individual experiments of 5–6 animals per group. (C) Viable pulmonary M. bovis BCG CFU growth curve data of co-infected (black) and BCG-only (clear) infected mice at days 14, 24 and 35 post BCG infection (D) Representative histological H&E stained lung sections captured at 10x magnification illustrating the differences in histopathology between BCG/T.muris co-infected, BCG-only infected, uninfected and T. muris-only infected BALB/c mice infected according to experimental design as shown in Figure 1A. (E) Pulmonary histopathological scoring was performed in a blinded fashion according to the degree of peribronchiolitis (b), perivasculitis (v), interstitial pneumonitis (i) and alveolitis (a) per lung. Average pulmonary scores of BALB/c mice infected according to experimental design as shown in Figure 1A. Groups included naive (circle), T. muris-only (diamond), BCG-only (triangle) and co-infected (square) mice. Data display mean ± SD, representing 2 individual experiments of 5–6 animals per group. P values <0.05 were considered statistically significant. (*p ≤ 0.05, ns = non significant).

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