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Table 2 Kinetic properties of TanLpl, TanLpa, and TanLpe a

From: Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus

Substarate

TanLpl

TanLpa

TanLpe

K m(mM)

k c at (s-1)

k cat/K m(s-1 · mM-1)

K m(mM)

k c at (s-1)

k cat/K m(s-1 · mM-1)

K m(mM)

k c at (s-1)

k cat/K m(s-1 · mM-1)

Methyl gallate (MG)

0.37 ± 0.04

46.02 ± 0.87

125.02 ± 15.43

0.50 ± 0.06

72.73 ± 3.34

145.12 ± 10.65

0.87 ± 0.41

15.95 ± 3.13

18.79 ± 3.08

Epicatechin gallate (ECg)

0.03 ± 0.02

1.49 ± 0.19

52.23 ± 25.64

0.06 ± 0.01

11.08 ± 0.44

195.30 ± 21.53

0.05 ± 0.01

0.42 ± 0.03

8.63 ± 1.17

Epigallocatechin gallate (EGCg)

0.10 ± 0.01

1.12 ± 0.03

11.68 ± 1.29

0.06 ± 0.01

14.29 ± 0.82

260.76 ± 46.52

0.06 ± 0.02

0.44 ± 0.02

7.25 ± 2.51

Catechin gallate (Cg)

0.05 ± 0.002

2.41 ± 0.10

53.65 ± 4.62

0.05 ± 0.005

8.1 ± 0.04

181.5 ± 27.71

0.08 ± 0.004

1.48 ± 0.11

19.22 ± 2.36

Gallocatechin gallate (GCg)

0.03 ± 0.008

0.89 ± 0.044

27.19 ± 6.28

0.06 ± 0.002

9.2 ± 0.09

154.68 ± 7.97

0.07 ± 0.002

1.12 ± 0.13

14.32 ± 1.95

Epigallocatechin-3-O-(3-O-methyl) gallate (EGCg3″Me)

0.04 ± 0.009

0.26 ± 0.04

6.04 ± 0.57

0.04 ± 0.004

0.35 ± 0.07

9.02 ± 2.28

0.005 ± 0.0009

0.06 ± 0.02

10.57 ± 1.33

  1. aAssays were carried out in triplicate and the results represent the means ± standard deviations.