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Table 2 Kinetic properties of TanLpl, TanLpa, and TanLpe a

From: Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus

Substarate TanLpl TanLpa TanLpe
K m(mM) k c at (s-1) k cat/K m(s-1 · mM-1) K m(mM) k c at (s-1) k cat/K m(s-1 · mM-1) K m(mM) k c at (s-1) k cat/K m(s-1 · mM-1)
Methyl gallate (MG) 0.37 ± 0.04 46.02 ± 0.87 125.02 ± 15.43 0.50 ± 0.06 72.73 ± 3.34 145.12 ± 10.65 0.87 ± 0.41 15.95 ± 3.13 18.79 ± 3.08
Epicatechin gallate (ECg) 0.03 ± 0.02 1.49 ± 0.19 52.23 ± 25.64 0.06 ± 0.01 11.08 ± 0.44 195.30 ± 21.53 0.05 ± 0.01 0.42 ± 0.03 8.63 ± 1.17
Epigallocatechin gallate (EGCg) 0.10 ± 0.01 1.12 ± 0.03 11.68 ± 1.29 0.06 ± 0.01 14.29 ± 0.82 260.76 ± 46.52 0.06 ± 0.02 0.44 ± 0.02 7.25 ± 2.51
Catechin gallate (Cg) 0.05 ± 0.002 2.41 ± 0.10 53.65 ± 4.62 0.05 ± 0.005 8.1 ± 0.04 181.5 ± 27.71 0.08 ± 0.004 1.48 ± 0.11 19.22 ± 2.36
Gallocatechin gallate (GCg) 0.03 ± 0.008 0.89 ± 0.044 27.19 ± 6.28 0.06 ± 0.002 9.2 ± 0.09 154.68 ± 7.97 0.07 ± 0.002 1.12 ± 0.13 14.32 ± 1.95
Epigallocatechin-3-O-(3-O-methyl) gallate (EGCg3″Me) 0.04 ± 0.009 0.26 ± 0.04 6.04 ± 0.57 0.04 ± 0.004 0.35 ± 0.07 9.02 ± 2.28 0.005 ± 0.0009 0.06 ± 0.02 10.57 ± 1.33
  1. aAssays were carried out in triplicate and the results represent the means ± standard deviations.